M-CSF/CSF1 Protein, Human, Recombinant (His & Avi)

CSF-1, also known as M-CSF , is a four alpha -helical-bundle cytokine that is the primary regulator of macrophage survival, proliferation and differentiation. CSF-1 is also essential for the survival and proliferation of osteoclast progenitors.CSF-1 is cytokine that plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes. M-CSF/CSF1 Protein, Human, Recombinant (His & Avi) is expressed in HEK293 mammalian cells with C-His-Avi tag. The predicted molecular weight is 21.3 kDa and the accession number is P09603-1.

规格	价格/CNY		数量
100 μg	￥ 4,440	5日内发货
500 μg	￥ 17,800	5日内发货
1 mg	￥ 29,600	5日内发货

规格

价格/CNY

货期

数量

100 μg

￥ 4,440

5日内发货

500 μg

￥ 17,800

5日内发货

1 mg

￥ 29,600

5日内发货

生物活性

1. Immobilized Human M-CSF, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Human M-CSF R, hFc Tag with the EC50 of 14.2ng/ml determined by ELISA (QC Test).2 . Human M-CSF, His Tag captured on CM5 Chip via Anti-his Antibody can bind Human M-CSF R, hFc Tag with an affinity constant of 0.24 nM as determined in SPR assay. 3. Measured in a cell proliferation assay using RAW264.7 cells. The ED50 for this effect is 2.5 - 7.0 ng/mL.

产品描述

种属	Human
表达系统	HEK293 Cells
标签	C-His-Avi
蛋白编号	P09603-1
别名	CSF-1, Lanimostim, M-CSF, MCSF, MGC31930, CSF1, 集落刺激因子
蛋白构建	Glu33-Asn190
蛋白纯度	> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
分子量	21.3 kDa (predicted). Due to glycosylation, the protein migrates to 28-34 kDa based on Tris-Bis PAGE result.

内毒素	< 1 EU/μg by the LAL method.
缓冲液	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS (pH 7.4). Typically, 8% trehalose is incorporated as a protective agent before lyophilization.
复溶方法	Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
存储	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
运输方式	In general, Lyophilized powders are shipping with blue ice.
研究背景	CSF-1, also known as M-CSF , is a four alpha -helical-bundle cytokine that is the primary regulator of macrophage survival, proliferation and differentiation. CSF-1 is also essential for the survival and proliferation of osteoclast progenitors.CSF-1 is cytokine that plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes.