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Lysozyme C Protein, Chicken, Recombinant(E53A)

产品编号 TMPH-00377

Lysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte-macrophage system and enhance the activity of immunoagents. Has bacteriolytic activity against M.luteus. Lysozyme C Protein, Chicken, Recombinant(E53A) is expressed in E. coli expression system. The predicted molecular weight is 14.4 kDa and the accession number is P00698.

Lysozyme C Protein, Chicken, Recombinant(E53A)

Lysozyme C Protein, Chicken, Recombinant(E53A)

产品编号 TMPH-00377
Lysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte-macrophage system and enhance the activity of immunoagents. Has bacteriolytic activity against M.luteus. Lysozyme C Protein, Chicken, Recombinant(E53A) is expressed in E. coli expression system. The predicted molecular weight is 14.4 kDa and the accession number is P00698.
规格价格库存数量
20 μg
¥ 3,280
20日内发货
100 μg
¥ 5,830
20日内发货
1 mg
¥ 16,800
20日内发货
大包装 & 定制
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实验操作小课堂
常见问题解答
抑制剂母液的储存方式?
母液配置完成后,一般储存于 -80℃,可以存 1 年以上。建议多分装几管,避免反复冻融,常用的存于 4 度,可放一周以上。
收到粉状试剂粘壁严重,如何处理?
建议您用离心机 3000 rpm下离心一会儿,将粉末收集到管底。
收到化合物后,是否需要称量后配制储备液?
对于小包装,如 1 mg、5 mg、10 mg 等,不建议二次称量。这样会导致化合物损耗,且无法估算。TargetMol 所有产品原装进口,精准称量,您收到后可直接添加相应量溶剂。 对于大包装,如果您需要分批次实验,可每次称量后配制储备液,建议您 10 mg 起称,称量的误差会小一些;当然条件允许,也可以先配制成高浓度母液,分装备用。
得到的杀死细胞的 IC50 与网页上 IC50 差距较大怎么办?
您所测试的IC50是指细胞的增殖抑制实验(半抑制率),细胞增殖抑制实验 IC50值,和网页上的靶点IC50其实是不同的意义。文献里和网页上 IC50其实往往是指对于靶点无细胞实验的抑制率,这样的实验一般是通过激酶或者蛋白纯化实验做出来的,nM 级别的浓度比较常见,但是细胞的增殖抑制实验 IC50 是指细胞的半致死率,同时牵涉到细胞的代谢以及渗透,一般浓度会高一些。 另外,同一个化合物对不同细胞模型的效果也是不同的,建议尝试增加孵育量、延长孵育时间。
Tween-20 可以用 Tween-80 替代吗?
不建议使用 Tween-20。相较于 Tween-20,Tween-80 的耐受性更好,Tween-80已被用作评估实验药物和毒物的行为影响的工具,且没有明显的副作用。 参考文献:Castro CA, Hogan JB, Benson KA, Shehata CW, Landauer MR. Behavioral effects of vehicles: DMSO, ethanol, Tween-20, Tween-80, and emulphor-620. Pharmacol Biochem Behav. 1995 Apr;50(4):521-6.
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产品信息

生物活性
Activity has not been tested. It is theoretically active, but we cannot guarantee it. If you require protein activity, we recommend choosing the eukaryotic expression version first.
产品描述
Lysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte-macrophage system and enhance the activity of immunoagents. Has bacteriolytic activity against M.luteus. Lysozyme C Protein, Chicken, Recombinant(E53A) is expressed in E. coli expression system. The predicted molecular weight is 14.4 kDa and the accession number is P00698.
种属
Chicken
表达系统
E. coli
标签Tag Free
蛋白编号P00698
别名
LYZ,Lysozyme C,Allergen Gal d IV,1,4-beta-N-acetylmuramidase C
氨基酸序列
KVFGRCELAAAMKRHGLDNYRGYSLGNWVCAAKFASNFNTQATNRNTDGSTDYGILQINSRWWCNDGRTPGSRNLCNIPCSALLSSDITASVNCAKKIVSDGNGMNAWVAWRNRCKGTDVQAWIRGCRL
蛋白构建
19-147 aa (E53A)
蛋白纯度
> 85% as determined by SDS-PAGE.
分子量14.4 kDa (predicted)
内毒素< 1.0 EU/μg of the protein as determined by the LAL method.
缓冲液If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
复溶方法
Reconstitute the lyophilized protein in sterile deionized water. The product concentration should not be less than 100 μg/mL. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
存储
Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
运输方式In general, Lyophilized powders are shipping with blue ice. Solutions are shipping with dry ice.
研究背景
Lysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte-macrophage system and enhance the activity of immunoagents. Has bacteriolytic activity against M.luteus.

参考文献

SCI 文献

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