LILRB4,also known as CD85k and LIR-5, ILT-3, is an approximately 60 kDa transmembrane glycoprotein that negatively regulates immune cell activation. Mature human ILT-3 consists of a 238 amino acid (aa) extracellular domain with two Ig-like domains, a 21 aa transmembrane segment, and a 168 aa cytoplasmic domain with 3 immunoreceptor tyrosine-based inhibitory motifs (ITIM).LILRB4 is receptor for class I MHC antigens. Recognizes a broad spectrum of HLA-A, HLA-B, HLA-C and HLA-G alleles. LILRB4/CD85k/ILT3 Protein, Human, Recombinant (His & Avi) is expressed in HEK293 mammalian cells with C-His-Avi tag. The predicted molecular weight is 28.6 kDa and the accession number is AAH26309.
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
100 μg | ¥ 2,960 | 5日内发货 | ||
500 μg | ¥ 11,800 | 5日内发货 | ||
1 mg | ¥ 19,700 | 5日内发货 |
生物活性 | 1. Immobilized Human LILRB4, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-LILRB4 Antibody, hFc Tag with the EC50 of 9.5ng/ml determined by ELISA (QC Test). 2. Human APOE4, hFc Tag captured on CM5 Chip via Protein A can bind Human LILRB4, His Tag with an affinity constant of 11.87 nM as determined in SPR assay. |
产品描述 | LILRB4,also known as CD85k and LIR-5, ILT-3, is an approximately 60 kDa transmembrane glycoprotein that negatively regulates immune cell activation. Mature human ILT-3 consists of a 238 amino acid (aa) extracellular domain with two Ig-like domains, a 21 aa transmembrane segment, and a 168 aa cytoplasmic domain with 3 immunoreceptor tyrosine-based inhibitory motifs (ITIM).LILRB4 is receptor for class I MHC antigens. Recognizes a broad spectrum of HLA-A, HLA-B, HLA-C and HLA-G alleles. LILRB4/CD85k/ILT3 Protein, Human, Recombinant (His & Avi) is expressed in HEK293 mammalian cells with C-His-Avi tag. The predicted molecular weight is 28.6 kDa and the accession number is AAH26309. |
种属 | Human |
表达系统 | HEK293 Cells |
标签 | C-His-Avi |
蛋白编号 | AAH26309 |
别名 | ILT3, ILT-3, HM18, LILRB4, CD85K, LIR5 |
蛋白构建 | Gln22-His257 |
蛋白纯度 | > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
分子量 | 28.6 kDa (predicted). Due to glycosylation, the protein migrates to 38-42 kDa based on Tris-Bis PAGE result. |
内毒素 | < 1 EU/μg by the LAL method. |
缓冲液 | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS (pH 7.4). Typically, 8% trehalose is incorporated as a protective agent before lyophilization. |
复溶方法 | Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing. |
存储 |
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted protein solutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
运输方式 |
In general, Lyophilized powders are shipping with blue ice. |
研究背景 | LILRB4,also known as CD85k and LIR-5, ILT-3, is an approximately 60 kDa transmembrane glycoprotein that negatively regulates immune cell activation. Mature human ILT-3 consists of a 238 amino acid (aa) extracellular domain with two Ig-like domains, a 21 aa transmembrane segment, and a 168 aa cytoplasmic domain with 3 immunoreceptor tyrosine-based inhibitory motifs (ITIM).LILRB4 is receptor for class I MHC antigens. Recognizes a broad spectrum of HLA-A, HLA-B, HLA-C and HLA-G alleles. |
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LILRB4/CD85k/ILT3 Protein, Human, Recombinant (His & Avi) ILT3 HM-18 LIR-5 HM 18 ILT-3 HM18 LILRB4 LILRB-4 LILRB 4 CD85K LIR 5 LIR5 ILT 3 recombinant recombinant-proteins proteins protein