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Influenza A H1N1 (A/California/04/2009) Hemagglutinin/HA0 Protein, Biotinylated

产品编号 TMPY-05577Influenza A H1N1 (A/California/04/2009) Hemagglutinin/HA0 Protein, Biotinylated
Influenza A H1N1 (A/California/04/2009) Hemagglutinin/HA0 Protein, Biotinylated is expressed in Baculovirus insect cells. The predicted molecular weight is 61.5 kDa.
规格价格库存数量
20 μg
¥ 5,100
5日内发货
大包装 & 定制
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如何稀释工作液?
根据您的工作液浓度,计算母液所需要稀释的倍数和所需母液的体积。建议用水、生理盐水、PBS等溶剂进行稀释,如果是细胞实验,可以使用培养基。 取适当的溶剂缓慢加入到母液中,直到达到您的工作液浓度,可以通过涡旋或者移液枪轻轻吹打帮助混匀。 我们的化合物大部分是脂溶性的,所以在使用 PBS 或者培养基等稀释工作液的时候,会有沉淀析出,这些都是正常现象,大多时候可以通过超声完全溶解。 工作液建议现配现用!
母液配置的计算方法?
(1) c=n/v=m/M/v 浓度=物质的量/体积=质量/相对分子质量/体积 (2) 官网产品详情页下方有溶液配制表格,可以参考计算好的加入溶剂体积
超声应使用什么样的频率?
频率建议使用常规的超声清洗频率即可,一般在20 kHz - 100 kHz。
收到化合物后,是否需要称量后配制储备液?
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期货产品没有纯度啊?这个纯度在哪里看
期货产品的话,是没有纯度信息,也没有质检信息的。有了现货之后我们会进行质检,测出试剂的纯度和结构等相关信息,并且展示在官网的活性描述上方。
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产品信息

生物活性
Activity testing is in progress. It is theoretically active, but we cannot guarantee it. If you require protein activity, we recommend choosing the eukaryotic expression version first.
产品描述
Influenza A H1N1 (A/California/04/2009) Hemagglutinin/HA0 Protein, Biotinylated is expressed in Baculovirus insect cells. The predicted molecular weight is 61.5 kDa.
种属
H1N1
表达系统
Baculovirus Insect Cells
标签Tag Free
别名
Harvey rat sarcoma viral oncogene homolog
蛋白构建
A DNA sequence encoding the Influenza A virus (A/California/04/2009 (H1N1)) hemagglutinin (Met1-Ile566), termed as HA was expressed. The purified protein was biotinylated in vitro. Predicted N terminal: Asp 18
蛋白纯度
> 85 % as determined by SDS-PAGE.
分子量61.5 kDa (predicted)
内毒素< 1.0 EU/μg of the protein as determined by the LAL method.
缓冲液Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
复溶方法
A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
存储
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
运输方式In general, Lyophilized powders are shipping with blue ice.
研究背景
The influenza viral Hemagglutinin (HA) protein is a homotrimer with a receptor binding pocket on the globular head of each monomer.HA has at least 18 different antigens. These subtypes are named H1 through H18.HA has two functions. Firstly, it allows the recognition of target vertebrate cells, accomplished through the binding to these cells' sialic acid-containing receptors. Secondly, once bound it facilitates the entry of the viral genome into the target cells by causing the fusion of the host endosomal membrane with the viral membrane. The influenza virus Hemagglutinin (HA) protein is translated in cells as a single protein, HA, or hemagglutinin precursor protein. For viral activation, hemagglutinin precursor protein (HA) must be cleaved by a trypsin-like serine endoprotease at a specific site, normally coded for by a single basic amino acid (usually arginine) between the HA1 and HA2 domains of the protein. After cleavage, the two disulfide-bonded protein domains produce the mature form of the protein subunits as a prerequisite for the conformational change necessary for fusion and hence viral infectivity.

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