IL-6 Protein, Human, Recombinant is expressed in E. coli expression system. The predicted molecular weight is 20.95 kDa and the accession number is P05231.
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
20 μg | ¥ 1,750 | 现货 | ||
100 μg | ¥ 4,430 | 5日内发货 | ||
200 μg | ¥ 6,910 | 5日内发货 | ||
500 μg | ¥ 12,420 | 5日内发货 |
生物活性 |
1. Immobilized Human IL-6 at 2 μg/mL (100 μL/well) can bind Human IL-6R hFc, the EC50 of Human IL-6R hFc is 3.0-10.0 ng/mL.
2. Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is 0.1-0.8 ng/mL.
3.Loaded Recombinant Human IL-6R Protein, His Tag on His1K Biosensor, can bind Recombinant Human IL-6 Protein, no Tag with an affinity constant of 0.165 µM as determined in BLI assay (Routinely tested).
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产品描述 | IL-6 Protein, Human, Recombinant is expressed in E. coli expression system. The predicted molecular weight is 20.95 kDa and the accession number is P05231. |
种属 | Human |
表达系统 | E. coli |
标签 | Tag Free |
蛋白编号 | P05231 |
别名 | BSF2, 白细胞介素, HSF, IL-6, IFNB2, interleukin 6, HGF, Interleukin-6, 白介素 |
蛋白构建 | The mature form of Human IL6 (NP_000591.1) (Val30-Met212) was expressed with an initial Met at the N-terminus. |
蛋白纯度 |
≥ 95 % as determined by SDS-PAGE
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分子量 | 20.95 kDa (predicted) |
内毒素 | < 10 EU/mg of the protein. |
缓冲液 | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization. |
复溶方法 | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
存储 |
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
运输方式 |
In general, Lyophilized powders are shipping with blue ice. |
研究背景 | Interleukin-6 (IL-6) is a multifunctional α-helical cytokine that regulates cell growth and differentiation of various tissues, which is known particularly for its role in the immune response and acute phase reactions. IL-6 protein is secreted by a variety of cell types including T cells and macrophages as a phosphorylated and variably glycosylated molecule. It exerts actions through its heterodimeric receptor composed of IL-6R that lacks the tyrosine/kinase domain and binds IL-6 with low affinity, and ubiquitously expressed glycoprotein 130 (gp130) that binds the IL-6. IL-6R complex with high affinity and thus transduces signals. IL-6 is also involved in hematopoiesis, bone metabolism, and cancer progression, and has been defined as an essential role in directing the transition from innate to acquired immunity.Cancer ImmunotherapyImmune CheckpointImmunotherapyTargeted Therapy |
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IL-6 Protein, Human, Recombinant BSF2 白细胞介素 HSF IL-6 IL6 IFNB2 interleukin 6 IFNB-2 HGF IL 6 Interleukin-6 IFNB 2 BSF 2 白介素 BSF-2 recombinant recombinant-proteins proteins protein