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IL-5 Protein, Human, Recombinant (HEK293)

产品编号 TMPY-07129IL-5 Protein, Human, Recombinant (HEK293)

IL-5 Protein, Human, Recombinant (HEK293) is expressed in HEK293 Cells. The accession number is P05113-1.

IL-5 Protein, Human, Recombinant (HEK293)

IL-5 Protein, Human, Recombinant (HEK293)

产品编号 TMPY-07129IL-5 Protein, Human, Recombinant (HEK293)
IL-5 Protein, Human, Recombinant (HEK293) is expressed in HEK293 Cells. The accession number is P05113-1.
规格价格库存数量
20 μg
¥ 1,750
期货
100 μg
¥ 4,420
期货
1 mg 待询 期货
大包装 & 定制
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1) 存储条件:重组蛋白产品收到后,如不立即使用,应当保存在 -20℃ 至 -80℃ 的条件下。冻干粉可稳定保存 12 个月以上,液体类产品在 -80℃ 条件下可保存 6-12 个月。已复溶的重组蛋白样品需尽快用完,如果不能在一周内用完,需在 -20℃ 至 -80℃ 保存,可保存 3 个月以上\n2) 避免反复融冻:重组蛋白产品应注意避免反复冻融,建议将重组蛋白产品分装成多份。分装冻存时,建议选择含有一定浓度载体蛋白的溶液或培养基复溶产品。产品浓度不低于 100 ug/mL,且每管的体积不小于 20 uL。
如何将冻干的重组蛋白配置为储备液?
在打开盖⼦之前,先使用 10000-12000 rpm 的转速进行离心,20-30 秒,将附着在管盖或管壁上的冻⼲粉收集到管底,以避免损失。大多数蛋白可通过加入无菌蒸馏水来重新溶解。如果需要除水以外的其他稀释剂,产品 COA 中会对其进行说明。一般储备液浓度建议不要低于 100 μg/mL,分装储存避免反复冻融。
为什么重组蛋白的体积和质量与预期不符?
蛋白冻干粉由蛋白原液冻干而来,冻干粉质量不等同于蛋白质量。由于冻⼲产品的体积大⼩受多种因素影响,包括冻⼲前缓冲液的组分、盐离⼦浓度以及产品本身的浓度等。冻⼲粉可能看上去很多,也可能肉眼不易观察到,这些都是正常现象,请您放心使用。在收到产品后,照常复溶、使用或分装储存即可。
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产品信息

生物活性
1. Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is typically 0.1-1 ng/mL.
2. Loaded IL-5R alpha/CD125 Protein, Human, Recombinant (Membrane-bound, His) (Cat#TMPY-07016) on HIS1K Biosensor, can bind IL-5 Protein, Human, Recombinant (HEK293) (Cat#TMPY-07129) with an affinity constant of 43.200 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested).
产品描述
IL-5 Protein, Human, Recombinant (HEK293) is expressed in HEK293 Cells. The accession number is P05113-1.
种属
Human
表达系统
HEK293 Cells
标签Tag Free
蛋白编号P05113-1
别名
TRF,IL-5,EDF
蛋白构建
A DNA sequence encoding the human IL5 (NP_000870.1) (Ile20-Ser134) was expressed.
蛋白纯度
> 95% as determined by SDS-PAGE.
分子量13.15 kDa (predicted)
内毒素< 1.0 EU per μg protein as determined by the LAL method.
缓冲液Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
复溶方法
Please refer to the lot-specific COA.
存储
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
运输方式In general, Lyophilized powders are shipping with blue ice.
研究背景
Interleukin 5 (IL-5) is a member of the interleukin family with a length of 115 amino acids. Interleukins are a group of cytokines (secreted proteins/signaling molecules) that were first seen to be expressed by white blood cells (leukocytes) and has been found in a wide variety of body cells. Interleukin 5 or IL-5 is produced by T helper-2 cells and mast cells. It helps to stimulate B cell growth and increase immunoglobulin secretion and is considered a key mediator in eosinophil activation. Interleukin 5 (IL-5) has long been associated with several allergic diseases, including allergic rhinitis and asthma. Growth in the number of circulating, airway tissue, and induced sputum eosinophils have been observed in patients with these diseases. IL-5 also had something with the terminally differentiated granulocyte eosinophils. IL-5 was originally found as an eosinophil colony-stimulating factor. It has been proved to be a major regulator of eosinophil accumulation in tissues and can modulate eosinophil behavior at every stage from maturation to survival.

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计算器

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