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IL-10 Protein, Feline, Recombinant

产品编号 TMPY-00525

IL-10 Protein, Feline, Recombinant is expressed in E. coli expression system. The predicted molecular weight is 18.9 kDa and the accession number is P55029.2.

IL-10 Protein, Feline, Recombinant

IL-10 Protein, Feline, Recombinant

产品编号 TMPY-00525
IL-10 Protein, Feline, Recombinant is expressed in E. coli expression system. The predicted molecular weight is 18.9 kDa and the accession number is P55029.2.
规格价格库存数量
20 μg
¥ 1,750
5日内发货
大包装 & 定制
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常见问题解答
抑制剂能否用于动物实验?
可以的,我们的抑制剂都可以用于动物/体内实验。但有些化合物还没有用于动物实验的文献,这种情况下,建议您先做预实验,确保研究的可行性。
抑制剂能否用于细胞实验?
可以的,我们的抑制剂都可以用于细胞/体外实验。但有些化合物还没有用于细胞实验的文献,这种情况下,建议您先做预实验,确保研究的可行性。
如果低于说明书给出的最高溶解度情况下,产品仍未溶解怎么办?
建议您使用一些辅助溶解方法,如水浴加热至 45℃ 或用超声震荡,加速粉末溶解。
准备进行动物实验,收到大包装粉末后,如何分装溶解?
根据配制出来的溶液是不是澄清的确定一次性能配多少:如果配制出来是澄清溶液,可以一次性多配制一些,储存于4度冰箱,大概一周配一次,放久了可能会失效;如果配制出来是混悬液,建议每次使用的时候都现配现用。
溶解度中写的“< 1 mg/mL refers to the slightly soluble or insoluble ”是什么意思?
这句话指的是,如果溶解度 < 1 mg/mL,那我们就认为它是微溶或不溶的,这是针对写的“DMSO:Insoluble”或“DMSO:slightly soluble”这类化合物的。
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产品信息

生物活性
1. Immobilized felineIL10at 10 μg/ml (100 μl/well) can bind Cynomolgus IL10RA-Fc , The EC50 of Cynomolgus IL10RA-Fc is 0.14-0.32 μg/ml. 2. Measured in a cell proliferation assay using MC/9-2 mouse mast cells. The ED50 for this effect is typically 0.1-0.6 ng/mL.
产品描述
IL-10 Protein, Feline, Recombinant is expressed in E. coli expression system. The predicted molecular weight is 18.9 kDa and the accession number is P55029.2.
种属
Feline
表达系统
E. coli
标签Tag Free
蛋白编号P55029.2
别名
interleukin 10
蛋白构建
A DNA sequence encoding the feline IL10 (P55029.2) (Ser19-Ile178) was expressed with an initial Met at the N-terminal. Predicted N terminal: Met
蛋白纯度
> 95 % as determined by SDS-PAGE
分子量18.9 kDa (predicted); 19 kDa (reducing conditions)
内毒素Please contact us for more information.
缓冲液Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
复溶方法
A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
存储
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
运输方式In general, Lyophilized powders are shipping with blue ice.
研究背景
IL-10 is an anti-inflammatory cytokine that belongs to the IL-10 family. It is produced by a variety of cell lines, including T-cells, macrophages, mast cells, and other cell types, while it is produced primarily by monocytes and to a lesser extent by lymphocytes. IL-10 is mainly expressed in monocytes and Type 2 T helper cells (TH2), mast cells, CD4+CD25+Foxp3+ regulatory T cells, and also in a certain subset of activated T cells and B cells. IL-10 has pleiotropic effects in immunoregulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. IL-10 can block NF-kappa B activity and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice suggested the function of this cytokine as an essential immunoregulator in the intestinal tract. The importance of interleukin 10 for counteracting excessive immunity in the human body is revealed by the fact that patients with Crohn's disease react favorably towards treatment with bacteria producing recombinant IL-10. IL-10 inhibits the synthesis of some cytokines, including IFN-gamma, IL-2, IL-3, TNF, and GM-CSF produced by activated macrophages and by helper T-cells. It also displays a potent ability to suppress the antigen-presentation capacity of antigen-presenting cells. However, it is also stimulatory towards certain T cells and mast cells and stimulates B cell maturation and antibody production.Cancer ImmunotherapyImmune CheckpointImmunotherapyTargeted Therapy

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