规格

价格

库存

数量

5 μg

¥ 222

现货

10 μg

¥ 342

现货

20 μg

¥ 546

现货

50 μg

¥ 1,050

现货

100 μg

¥ 1,750

现货

200 μg

¥ 3,080

5日内发货

500 μg

¥ 6,530

5日内发货

生物活性

产品描述	IL-18BP Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 19 kDa and the accession number is A0A024R5G2.
生物活性	1. Measured by its ability to bind biotinylated Cynomolgus IL18 in functional ELISA. 2. Measured by its ability to inhibit the IL-18-induced response of KG‑1 human acute myelogenous leukemia cells. The ED50 for this effect is 0.05-0.35μg/mL in the presence of 50 ng/mL of recombinant human IL-18.
研究背景	Interleukin-18-binding protein (IL-18BP) is a constitutively expressed and secreted protein. IL-18BP is a cytokine receptor that belongs to the interleukin 1 receptor family. This receptor specifically binds interleukin 18 (IL18) and is essential for IL18 mediated signal transduction. IFN-alpha and IL12 are reported to induce the expression of this receptor in NK and T cells. This gene along with four other members of the interleukin 1 receptor family, including IL1R2, IL1R1, ILRL2 (IL-1Rrp2), and IL1RL1 (T1/ST2), form a gene cluster on chromosome 2q. The adjacently located family members IL18 Receptor 1 (IL18R1) and IL18 receptor accessory protein (IL18RAP) may also be important in the development of asthma and atopy. IL-18 binding protein (IL-18BP) was only moderately elevated, resulting in a high level of biologically active free IL-18 in HPS. A severe IL-18/IL-18BP imbalance results in Th-1 lymphocyte and macrophage activation, which escapes control by NK-cell cytotoxicity and may allow for secondary HPS in patients with underlying diseases.
种属	Human
表达系统	HEK293 Cells
标签	C-His
蛋白编号	O95998
蛋白构建	A DNA sequence encoding the human IL18BPa (NP_001034748.1) (Met1-Gly194) was expressed with the polyhistidine tag at the C-terminus. Predicted N terminal: Thr 31
蛋白纯度	> 85 % as determined by SDS-PAGE
蛋白性状	Lyophilized powder
缓冲液	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
复溶方法	Reconstituted with sterile deionized water to 0.25 mg/mL. Reconstitution conditions may vary depending on the lot.
别名	interleukin 18 binding protein, IL18BPa
内毒素	< 1.0 EU/μg of the protein as determined by the LAL method.

化学信息

分子量	19 kDa (predicted); 40.3 kDa (reducing condition, due to glycosylation)

储存&溶解度

运输方式

In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.

存储

It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

实际储存温度请以COA为准

IL-18BP Protein, Human, Recombinant (His)

IL-18BP Protein, Human, Recombinant (His)

产品介绍

引用文献

计算器

剂量转换

技术支持