生物活性

产品描述	IL-33 Protein, Human, Recombinant (His) is expressed in HEK293 Cells. The accession number is NP_254274.1.
生物活性	Immobilized Human IL-1RL1, hFc Tag at 1 μg/ml (100 μl/well) on the plate. Dose response curve for Human IL-33, His Tag with the EC50 of 1.93 μg/ml determined by ELISA (QC Test).
研究背景	Interleukin-33 (IL-33) is a cytokine belonging to the IL-1 family, playing a role in inflammatory, infectious and autoimmune diseases and expressed in the cellular nucleus in several tissues. High levels of IL-33 are expressed in epithelial barrier tissues and endothelial barriers. ST2 is a receptor for IL-33, expressed selectively on a subset of Th2 cells, mediating some of their functions.
种属	Human
表达系统	HEK293 Cells
标签	N-His
蛋白编号	NP_2542741
蛋白构建	His109-Thr270
蛋白纯度	> 95% as determined by Bis-Tris PAGE; > 90% as determined by HPLC
缓冲液	Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
复溶方法	Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
内毒素	< 1 EU/μg of the protein as determined by the LAL method.

化学信息

分子量	19.43 kDa (Predicted); 26-30 kDa (Reducing condition, due to glycosylation)

储存&溶解度

运输方式

In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.

存储

It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

实际储存温度请以COA为准

IL-33 Protein, Human, Recombinant (His)

IL-33 Protein, Human, Recombinant (His)

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