生物活性

产品描述	IL-25/IL17E Protein, Cynomolgus, Recombinant (His) is expressed in HEK293 Cells. The accession number is G7P9V9.
生物活性	Immobilized Cynomolgus IL-25, His Tag at 0.5 μg/ml (100 μl/well) on the plate. Dose response curve for Anti-IL-25 Antibody, hFc Tag with the EC50 of 3.8 ng/ml determined by ELISA (QC Test).
研究背景	IL-25, also known as IL-17E, is a member of the IL-17 cytokine family mostly produced by epithelial cells and innate immune cells. After binding to the IL-17RB/IL-17RA complex, IL-25 induces downstream signaling responses in epithelial cells and type 2 lymphocytes, which initiates, propagates, and sustains type 2 immunity. The function of IL-25 in allergic diseases such as asthma has been well established, and now also is extended to diseases such as inflammatory bowel disease and cancer.
种属	Cynomolgus
表达系统	HEK293 Cells
标签	C-His
蛋白编号	G7P9V9
蛋白构建	Tyr33-Gly176
蛋白纯度	> 95% as determined by Bis-Tris PAGE; > 90% as determined by HPLC
缓冲液	Lyophilized from 0.22 μm filtered solution in PBS, 8% trehalose (pH 7.4).
复溶方法	Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
内毒素	< 0.1 EU/μg of the protein as determined by the LAL method.

化学信息

分子量	18.18 kDa (Predicted); 27-37 kDa (Reducing condition, due to glycosylation)

储存&溶解度

运输方式

In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.

存储

It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

实际储存温度请以COA为准

IL-25/IL17E Protein, Cynomolgus, Recombinant (His)

IL-25/IL17E Protein, Cynomolgus, Recombinant (His)

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