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IL-1 beta/IL-1F2 Protein, Cynomolgus, Recombinant (His & Avi), Biotinylated is expressed in E. coli. The accession number is A0A2K5VDC7.
IL-1 beta/IL-1F2 Protein, Cynomolgus, Recombinant (His & Avi), Biotinylated is expressed in E. coli. The accession number is A0A2K5VDC7.


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| 产品描述 | IL-1 beta/IL-1F2 Protein, Cynomolgus, Recombinant (His & Avi), Biotinylated is expressed in E. coli. The accession number is A0A2K5VDC7. |
| 生物活性 | Immobilized Human IL-1R2, hFc Tag at 5 μg/ml (100 μl/well) on the plate. Dose response curve for Biotinylated Cynomolgus IL-1 Beta, His Tag with the EC50 of 15.3 ng/ml determined by ELISA (QC Test). |
| 研究背景 | Interleukin-1 beta (IL-1β) is induced by inflammatory signals in a broad number of immune cell types. IL-1β (and IL-18) are the only cytokines which are processed by caspase-1 after inflammasome-mediated activation. IL-1 signaling activates innate immune cells including antigen presenting cells, and drives polarization of CD4 T cells towards T helper type (Th) 1 and Th17 cells. |
| 种属 | Cynomolgus |
| 表达系统 | E. coli |
| 标签 | N-His-Avi |
| 蛋白编号 | A0A2K5VDC7 |
| 蛋白构建 | Ala117-Ser268 |
| 蛋白纯度 | > 95% as determined by Bis-Tris PAGE; > 95% as determined by HPLC |
| 缓冲液 | Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| 复溶方法 | Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing. |
| 内毒素 | < 1 EU/μg of the protein as determined by the LAL method. |
| 分子量 | 20.14 kDa (Predicted); 22-24 kDa (Reducing condition) |
| 运输方式 | In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice. |
| 存储 | It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. 实际储存温度请以COA为准 |
对于不同动物的给药剂量换算,您也可以参考 更多