IgG1 hFc Protein, Human, Recombinant, Biotinylated is expressed in HEK293 mammalian cells with C-Avi tag. The predicted molecular weight is 33 KDa and the accession number is P01857.
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
20 μg | ¥ 2,260 | 5日内发货 | ||
100 μg | ¥ 6,130 | 5日内发货 |
生物活性 | 1. Loaded Biotinylated Human IgG1 Fc-His-Avi on SA Biosensor, can bind Protein G-Fc with an affinity constant of <1 pM as determined in BLI assay. (Regularly tested) 2. Immobilized Biotinylated Human IgG1 Fc at 2μg/ml (100 μl/well) can bind Recombinant Protein G. The ED50 of Recombinant Protein G is 49.41 ng/ml. (Regularly tested) |
产品描述 | IgG1 hFc Protein, Human, Recombinant, Biotinylated is expressed in HEK293 mammalian cells with C-Avi tag. The predicted molecular weight is 33 KDa and the accession number is P01857. |
种属 | Human |
表达系统 | Human Cells |
标签 | C-Avi |
蛋白编号 | P01857 |
别名 | g gamma-1 chain C region, g γ-1 chain C region, IGHG1 |
氨基酸序列 | Asp104-Lys330(Asp239Glu,Leu241Glu) |
蛋白构建 | Biotinylated Recombinant Human Immunoglobulin G1 Fc is produced by our Mammalian expression system and the target gene encoding Asp104-Lys330(Asp239Glu,Leu241Glu) is expressed with a Avi tag at the C-terminus. |
蛋白纯度 | Greater than 95% as determined by reducing SDS-PAGE. (QC verified) |
分子量 | 33 KDa, reducing conditions |
内毒素 | Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test. |
缓冲液 | Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. |
复溶方法 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
存储 |
Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
运输方式 |
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature listed below. |
研究背景 | As a monomeric immunoglobulin that is predominately involved in the secondary antibody response and the only isotype that can pass through the human placenta, Immunoglobulin G (IgG) is synthesized and secreted by plasma B cells, and constitutes 75% of serum immunoglobulins in humans. IgG antibodies protect the body against the pathogens by agglutination and immobilization, complement activation, toxin neutralization, as well as the antibody-dependent cell-mediated cytotoxicity (ADCC). IgG tetramer contains two heavy chains (50 kDa ) and two light chains (25 kDa) linked by disulfide bonds, that is the two identical halves form the Y-like shape. IgG is digested by pepsin proteolysis into Fab fragment (antigen-binding fragment) and Fc fragment ("crystallizable" fragment). IgG1 is most abundant in serum among the four IgG subclasses (IgG1, 2, 3 and 4) and binds to Fc receptors (FcγR ) on phagocytic cells with high affinity. |
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IgG1 hFc Protein, Human, Recombinant, Biotinylated g gamma-1 chain C region g γ-1 chain C region IGHG1 IGHG 1 IGHG-1 recombinant recombinant-proteins proteins protein