生物活性

产品描述	IgE Protein, Human, Recombinant (His & Avi), Biotinylated is expressed in HEK293 Cells. The accession number is P01854-1.
生物活性	Immobilized Human Fc epsilon RI alpha, hFc Tag at 1 μg/ml (100 μl/well) on the plate. Dose response curve for Biotinylated Human IgE, His Avi Tag with the EC50 of 10.8 ng/ml determined by ELISA (QC Test).
研究背景	Immunoglobulin E (IgE) is well known for its role in allergic disease, the manifestations of which are mediated through its two Fc receptors, FcεRI and CD23 (FcεRII). IgE and its interactions with these receptors are therefore potential targets for therapeutic intervention, and exciting progress has been made in this direction. Furthermore, recent structural studies of IgE-Fc, the two receptors, and of their complexes, have revealed a remarkable degree of plasticity at the IgE-CD23 interface and an even more remarkable degree of dynamic flexibility within the IgE molecule.
种属	Human
表达系统	HEK293 Cells
标签	C-His-Avi
蛋白编号	P01854-1
蛋白构建	Cys209-Lys428
蛋白纯度	> 95% as determined by Bis-Tris PAGE; > 95% as determined by HPLC
缓冲液	Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
复溶方法	Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
内毒素	< 1 EU/μg of the protein as determined by the LAL method.

化学信息

分子量	27.8 kDa (Predicted); 32-38 kDa (Reducing condition, due to glycosylation)

储存&溶解度

运输方式

In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.

存储

It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

实际储存温度请以COA为准

IgE Protein, Human, Recombinant (His & Avi), Biotinylated

IgE Protein, Human, Recombinant (His & Avi), Biotinylated

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