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SARS-CoV-2 Guanine-N7 methyltransferase Protein (His)

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货号 TMPJ-01429

别名 SARS-CoV 2 nsp14, SARS-CoV 2 ExoN, Guanine-N7 methyltransferase

The nonstructural protein (nsp) 14 of SARS-CoV 2 was identified as a cap (guanine-N7)-methyltransferase (N7-MTase). Nsp14 of coronaviruses two different activities: an exoribonuclease activity acting on both ssRNA and dsRNA in a 3' to 5' direction and a N7-guanine methyltransferase activity. It may be involved in the proof-reading ability during the viral RNA replication and transcription. GTP, dGTP as well as cap analogs GpppG, GpppA and m7GpppG could be methylated by nsp14.

SARS-CoV-2 Guanine-N7 methyltransferase Protein (His)
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SARS-CoV-2 Guanine-N7 methyltransferase Protein (His)

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货号 TMPJ-01429 别名 SARS-CoV 2 nsp14, SARS-CoV 2 ExoN, Guanine-N7 methyltransferase

The nonstructural protein (nsp) 14 of SARS-CoV 2 was identified as a cap (guanine-N7)-methyltransferase (N7-MTase). Nsp14 of coronaviruses two different activities: an exoribonuclease activity acting on both ssRNA and dsRNA in a 3' to 5' direction and a N7-guanine methyltransferase activity. It may be involved in the proof-reading ability during the viral RNA replication and transcription. GTP, dGTP as well as cap analogs GpppG, GpppA and m7GpppG could be methylated by nsp14.

规格价格库存数量
5 μg
¥ 693
6-8日内发货
10 μg
¥ 1,170
6-8日内发货
20 μg
¥ 1,880
5日内发货
50 μg
¥ 3,550
5日内发货
100 μg
¥ 5,860
5日内发货
200 μg
¥ 9,690
5日内发货
500 μg
¥ 18,900
5日内发货
1 mg
¥ 28,500
5日内发货
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产品介绍

生物活性
产品描述
The nonstructural protein (nsp) 14 of SARS-CoV 2 was identified as a cap (guanine-N7)-methyltransferase (N7-MTase). Nsp14 of coronaviruses two different activities: an exoribonuclease activity acting on both ssRNA and dsRNA in a 3' to 5' direction and a N7-guanine methyltransferase activity. It may be involved in the proof-reading ability during the viral RNA replication and transcription. GTP, dGTP as well as cap analogs GpppG, GpppA and m7GpppG could be methylated by nsp14.
生物活性
Activity has not been tested. It is theoretically active, but we cannot guarantee it. If you require protein activity, we recommend choosing the eukaryotic expression version first.
研究背景
The nonstructural protein (nsp) 14 of SARS-CoV 2 was identified as a cap (guanine-N7)-methyltransferase (N7-MTase). Nsp14 of coronaviruses two different activities: an exoribonuclease activity acting on both ssRNA and dsRNA in a 3' to 5' direction and a N7-guanine methyltransferase activity. It may be involved in the proof-reading ability during the viral RNA replication and transcription. GTP, dGTP as well as cap analogs GpppG, GpppA and m7GpppG could be methylated by nsp14.
种属
SARS-CoV-2
表达系统
E. coli
标签N-6xHis
蛋白编号YP_009725309.1
氨基酸序列Ala1-Gln527
蛋白构建Ala1-Gln527
缓冲液Supplied as a 0.2 μm filtered solution of 20 mM PB, 100 mM NaCl, 2 mM DTT, 20% Glycerol, 0.1% TritonX-100, pH 6.0.
别名SARS-CoV 2 nsp14, SARS-CoV 2 ExoN, Guanine-N7 methyltransferase
化学信息
分子量60 KDa (reducing condition)
储存&溶解度
运输方式In general, Lyophilized powders are shipping with blue ice. Solutions are shipping with dry ice.
存储Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

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技术支持

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