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GM-CSFR alpha Protein, Human, Recombinant (His & Avi), Biotinylated

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GM-CSFR alpha Protein, Human, Recombinant (His & Avi), Biotinylated

产品编号 TMPK-00254

In the first trimester, the GM-CSF-R was found to be present on villous cytotrophoblast and all populations of extravillous trophoblast. Expression by villous syncytiotrophoblast was weak or absent, but this increased markedly by term. GM-CSF-R were also expressed by fetal Hofbauer cells within the mesenchyme of the chorionic villi and by uterine glandular epithelium and decidual macrophages within maternal decidua. GM-CSFR alpha Protein, Human, Recombinant (His & Avi), Biotinylated is expressed in HEK293 mammalian cells with C-His-Avi tag. The predicted molecular weight is 37.7 kDa and the accession number is P15509-1.

GM-CSFR alpha Protein, Human, Recombinant (His & Avi), Biotinylated

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产品编号 TMPK-00254

规格	价格	库存
5 μg	¥ 568	6-8日内发货
10 μg	¥ 928	6-8日内发货
20 μg	¥ 1,530	5日内发货
50 μg	¥ 2,970	5日内发货
100 μg	¥ 5,180	5日内发货
200 μg	¥ 9,390	5日内发货
500 μg	¥ 20,700	5日内发货

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产品信息

生物活性	1. Immobilized Anti-GM-CSF R alpha Antibody, hFc Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human GM-CSF R alpha, His Tag with the EC50 of 7.7ng/ml determined by ELISA. 2. Biotinylated Human GM-CSF R alpha, His-Avi Tag captured on CM5 Chip via Anti-His Antibody can bind Human GM-CSF, No Tag with an affinity constant of 1.68 nM as determined in SPR assay.
产品描述	In the first trimester, the GM-CSF-R was found to be present on villous cytotrophoblast and all populations of extravillous trophoblast. Expression by villous syncytiotrophoblast was weak or absent, but this increased markedly by term. GM-CSF-R were also expressed by fetal Hofbauer cells within the mesenchyme of the chorionic villi and by uterine glandular epithelium and decidual macrophages within maternal decidua. GM-CSFR alpha Protein, Human, Recombinant (His & Avi), Biotinylated is expressed in HEK293 mammalian cells with C-His-Avi tag. The predicted molecular weight is 37.7 kDa and the accession number is P15509-1.
种属	Human
表达系统	HEK293 Cells
标签	C-His-Avi
蛋白编号	P15509-1
别名	集落刺激因子,SMDP4,GMR-α,GMR-alpha,GMR,GMCSFR-α,GM-CSF-R-α,GMCSFR-alpha,GM-CSF-R-alpha,GMCSFR,GM-CSF R α Protein,CSF2RY,CSF2RX,CSF2RAY,CSF2RAX,CSF2RA,CSF2R,CDw116,CD116
蛋白构建	Glu23-Gly320
蛋白纯度	> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
分子量	37.7 kDa (predicted). Due to glycosylation, the protein migrates to 68-75 kDa based on Tris-Bis PAGE result.
内毒素	< 1 EU/μg by the LAL method.
蛋白性状	Lyophilized powder
缓冲液	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS (pH 7.4). Typically, 8% trehalose is incorporated as a protective agent before lyophilization.
复溶方法	Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
存储	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
运输方式	In general, Lyophilized powders are shipping with blue ice.
研究背景	In the first trimester, the GM-CSF-R was found to be present on villous cytotrophoblast and all populations of extravillous trophoblast. Expression by villous syncytiotrophoblast was weak or absent, but this increased markedly by term. GM-CSF-R were also expressed by fetal Hofbauer cells within the mesenchyme of the chorionic villi and by uterine glandular epithelium and decidual macrophages within maternal decidua.