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GHR/Growth Hormone R Protein, Pig, Recombinant (His)

产品编号 TMPH-03112

Receptor for pituitary gland growth hormone involved in regulating postnatal body growth. On ligand binding, couples to, and activates the JAK2/STAT5 pathway.; The soluble form (GHBP) acts as a reservoir of growth hormone in plasma and may be a modulator/inhibitor of GH signaling. GHR Protein, Pig, Recombinant (His) is expressed in E. coli expression system with C-6xHis tag. The predicted molecular weight is 30.3 kDa and the accession number is P19756.

GHR/Growth Hormone R Protein, Pig, Recombinant (His)

GHR/Growth Hormone R Protein, Pig, Recombinant (His)

产品编号 TMPH-03112
Receptor for pituitary gland growth hormone involved in regulating postnatal body growth. On ligand binding, couples to, and activates the JAK2/STAT5 pathway.; The soluble form (GHBP) acts as a reservoir of growth hormone in plasma and may be a modulator/inhibitor of GH signaling. GHR Protein, Pig, Recombinant (His) is expressed in E. coli expression system with C-6xHis tag. The predicted molecular weight is 30.3 kDa and the accession number is P19756.
规格价格库存数量
20 μg
¥ 1,800
20日内发货
100 μg
¥ 3,760
20日内发货
1 mg
¥ 16,000
20日内发货
大包装 & 定制
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实验操作小课堂
常见问题解答
抑制剂加 DMSO 配成母液之后,能否放在常温的环境保存1周?
不建议这么做,母液配置好分装保存,建议存于 -20℃ 或 -80℃,尽量避免反复冻融。如果您订购的规格很大,可以考虑分装粉末保存,固体粉末是比较稳定的,-20℃ 可以保存三年。
超声应使用什么样的频率?
频率建议使用常规的超声清洗频率即可,一般在20 kHz - 100 kHz。
是否能直接用缓冲液对抑制剂 DMSO 母液做梯度稀释?
大部分情况下,都是可以溶解的。但有时,有机试剂直接加入水相介质时会析出。建议将抑制剂先以DMSO做梯度稀释,再将经过稀释的抑制剂加入缓冲液或细胞培养基。有些抑制剂甚至只有在其工作浓度下才能溶于水相。 比如细胞实验中希望终浓度为 1 μM 的话,可以把 10 mM 的 DMSO 母液用 DMSO 稀释到 1 mM,再吸 2 μL 加入到 2 mL 的生理盐水/PBS/细胞培液,终浓度即为 1 μM。 为避免药物析出,稀释前,可将母液和培养基 37℃ 预热,避免温度低造成严重析出。若稀释过程中出现化合物析出的情况,建议您采用超声加热的方法使其复溶。
PEG300 可以用 PEG400 替代吗?
PEG300 具有适度的粘稠度,有利于药物的溶解和给药过程,同时也能够提供良好的耐受性,不会对动物造成不良影响。PEG400 可以替代 PEG300。 PEG600 不是很建议,因为熔点接近常温。
准备进行动物实验,收到大包装粉末后,如何分装溶解?
根据配制出来的溶液是不是澄清的确定一次性能配多少:如果配制出来是澄清溶液,可以一次性多配制一些,储存于4度冰箱,大概一周配一次,放久了可能会失效;如果配制出来是混悬液,建议每次使用的时候都现配现用。
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产品信息

生物活性
Activity has not been tested. It is theoretically active, but we cannot guarantee it. If you require protein activity, we recommend choosing the eukaryotic expression version first.
产品描述
Receptor for pituitary gland growth hormone involved in regulating postnatal body growth. On ligand binding, couples to, and activates the JAK2/STAT5 pathway.; The soluble form (GHBP) acts as a reservoir of growth hormone in plasma and may be a modulator/inhibitor of GH signaling. GHR Protein, Pig, Recombinant (His) is expressed in E. coli expression system with C-6xHis tag. The predicted molecular weight is 30.3 kDa and the accession number is P19756.
种属
Sus scrofa (Pig)
表达系统
E. coli
标签C-6xHis
蛋白编号P19756
别名
Somatotropin receptor,Growth hormone receptor,GHR,GH receptor
氨基酸序列
FSGSEATPAVLVRASQSLQRVHPGLETNSSGKPKFTKCRSPELETFSCHWTDGVRHGLQSPGSIQLFYIRRSTQEWTQEWKECPDYVSAGENSCYFNSSYTSIWIPYCIKLTSNGGTVDQKCFSVEEIVQPDPPIGLNWTLLNISLTGIHADIQVRWEPPPNADVQKGWIVLEYELQYKEVNETQWKMMDPVLSTSVPVYSLRLDKEYEVRVRSRQRNSEKYGEFSEVLYVTLPQMSPFACEEDFR
蛋白构建
19-264 aa
蛋白纯度
> 85% as determined by SDS-PAGE.
分子量30.3 kDa (predicted)
内毒素< 1.0 EU/μg of the protein as determined by the LAL method.
缓冲液Tris-based buffer, 50% glycerol
复溶方法
A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
存储
Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
运输方式In general, Lyophilized powders are shipping with blue ice. Solutions are shipping with dry ice.
研究背景
Receptor for pituitary gland growth hormone involved in regulating postnatal body growth. On ligand binding, couples to, and activates the JAK2/STAT5 pathway.; The soluble form (GHBP) acts as a reservoir of growth hormone in plasma and may be a modulator/inhibitor of GH signaling.

SCI 文献

计算器

  • 复溶 计算器
  • 重组蛋白稀释 计算器
  • 比活力 计算器

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