生物活性

产品描述	FGF-1 Protein, Human, Recombinant (His & Avi), Biotinylated is expressed in E. coli. The accession number is P05230-1.
生物活性	Immobilized Human FGFR1 alpha (IIIc), His Tag at 5 μg/ml (100 μl/well) on the plate. Dose response curve for Biotinylated Human FGF1, His-Avi Tag with the EC50 of 100.7 ng/ml determined by ELISA (QC Test).
研究背景	Fibroblast Growth Factor 1 (Fgf1), also known as acidic FGF (aFGF), is involved in the regulation of various biological processes, ranging from development to disease pathogenesis. It is a single chain polypeptide and is highly expressed in adult brain and kidney tissues. Its expression has been shown to be directed by multiple tissue-specific promoters, which generate transcripts of varying lengths.
种属	Human
表达系统	E. coli
标签	N-His-Avi
蛋白编号	P05230-1
蛋白构建	Phe16-Asp155
蛋白纯度	> 95% as determined by Bis-Tris PAGE
缓冲液	Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
复溶方法	Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
内毒素	< 1 EU/μg of the protein as determined by the LAL method.

化学信息

分子量	18.74 kDa (Predicted); 20-24 kDa (Reducing condition, due to glycosylation)

储存&溶解度

运输方式

In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.

存储

It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

实际储存温度请以COA为准

FGF-1 Protein, Human, Recombinant (His & Avi), Biotinylated

FGF-1 Protein, Human, Recombinant (His & Avi), Biotinylated

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