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FcRn Protein, Rat, Recombinant (His & Avi), Biotinylated is expressed in HEK293 Cells. The accession number is P13599(FCGRT)&P07151(B2M).
FcRn Protein, Rat, Recombinant (His & Avi), Biotinylated is expressed in HEK293 Cells. The accession number is P13599(FCGRT)&P07151(B2M).


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| 产品描述 | FcRn Protein, Rat, Recombinant (His & Avi), Biotinylated is expressed in HEK293 Cells. The accession number is P13599(FCGRT)&P07151(B2M). |
| 生物活性 | Biotinylated Rat FcRn, His Tag captured on CM5 Chip via Anti-His Antibody can bind Trastuzumab with an affinity constant of 5.08 nM as determined in SPR assay (Biacore T200) (QC Test). |
| 研究背景 | The neonatal Fc receptor (FCRN) is an approximately 45 kDa transmembrane glycoprotein with structural homology to MHC class I proteins. It is widely expressed in endothelial and epithelial cells and plays an important role in IgG homeostasis and antigen presentation by dendritic cells.FCGRT & B2M heterodimer protein (FcRn complex) consist of two subunits: p51, and p14 , and forms an MHC class I-like heterodimer. |
| 种属 | Rat |
| 表达系统 | HEK293 Cells |
| 标签 | C-His-Avi |
| 蛋白编号 | P13599 (FCGRT) & P07151 (B2M) |
| 蛋白构建 | Ala23-Ser298 (FCGRT) and Ile21-Met119 (B2M) |
| 蛋白纯度 | > 95% as determined by Bis-Tris PAGE; > 95% as determined by HPLC |
| 缓冲液 | Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| 复溶方法 | Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing. |
| 内毒素 | < 1 EU/μg of the protein as determined by the LAL method. |
| 分子量 | 33.9 kDa (FCGRT) and 11.63 kDa (B2M) (Predicted); 45-60 kDa (FCGRT) and 12-14 kDa (B2M) (Reducing condition, due to glycosylation) |
| 运输方式 | In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice. |
| 存储 | It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. 实际储存温度请以COA为准 |
对于不同动物的给药剂量换算,您也可以参考 更多