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ENTPD5 Protein, Human, Recombinant (His)

产品编号 TMPY-02758

别名: NTPDase-5, ectonucleoside triphosphate diphosphohydrolase 5, CD39L4, PCPH

ENTPD5 Protein, Human, Recombinant (His) is expressed in Baculovirus insect cells with His tag. The predicted molecular weight is 47 kDa and the accession number is O75356.

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ENTPD5 Protein, Human, Recombinant (His)

规格	价格/CNY		货期	数量
50 μg	￥ 3,820		5日内发货

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生物活性

技术参数

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参考文献

生物活性	Measured by its ability to hydrolyze the 5’phosphate groups from the substrate guanosine5’diphosphate (GDP). The specific activity is >4,000 pmoles/min/μg.
产品描述	ENTPD5 Protein, Human, Recombinant (His) is expressed in Baculovirus insect cells with His tag. The predicted molecular weight is 47 kDa and the accession number is O75356.

种属	Human
表达系统	Baculovirus Insect Cells
标签	C-His
蛋白编号	O75356
别名	NTPDase-5, ectonucleoside triphosphate diphosphohydrolase 5, CD39L4, PCPH
蛋白构建	The Human ENTPD5 (O75356) (Met 1-His 428) was fused with a polyhistidine tag at the C-terminus.
蛋白纯度	> 90 % as determined by SDS-PAGE
分子量	47 kDa (predicted)

内毒素	< 1.0 EU/μg of the protein as determined by the LAL method.
缓冲液	Lyophilized from a solution filtered through a 0.22 μm filter, containing 20 mM Tris, 500 mM NaCl, pH 7.4, 10% gly. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
复溶方法	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
存储	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
运输方式	In general, Lyophilized powders are shipping with blue ice.
研究背景	Ectonucleoside triphosphate diphosphohydrolase 5 (ENTPD5), also known as CD39 antigen-like 4, ER-UDPase, Guanosine-diphosphatase ENTPD5, Nucleoside diphosphatase Uridine-diphosphatase ENTPD5. This hydrolase is expressed in response to phosphoinositide 3-kinase (PI3K) signaling. Activation of PI3K results in FOXO phosphorylation by AKT1 and loss of ENTPD5 transcriptional repression. It is Up-regulated in PTEN-deficient cells. Uridine diphosphatase (UDPase) that promotes protein N-glycosylation and ATP level regulation.ENTPD5 promotes protein N-glycosylation and folding in the endoplasmic reticulum, as well as elevated ATP consumption in the cytosol via an ATP hydrolysis cycle. Together with CMPK1 and AK1, ENTPD5 constitutes an ATP hydrolysis cycle that converts ATP to AMP and results in a compensatory increase in aerobic glycolysis. ENTPD5 also hydrolyzes GDP and IDP but not any other nucleoside di-, mono- or triphosphates, nor thiamine pyrophosphate. This enzyme Plays a key role in the AKT1-PTEN signaling pathway by promoting glycolysis in proliferating cells in response to phosphoinositide 3-kinase (PI3K) signaling.

参考文献

1. Villar J,et al.(2009) PCPH/ENTPD5 expression confers to prostate cancer cells resistance against cisplatin-induced apoptosis through protein kinase Calpha-mediated Bcl-2 stabilization. Cancer Res. 69(1): 102-10. 2. Fang M,et al.(2010) The ER UDPase ENTPD5 promotes protein N-glycosylation, the Warburg effect, and proliferation in the PTEN pathway. Cell. 143(5): 711-24. 3. Paez JG,et al.(2001) Identity between the PCPH proto-oncogene and the CD39L4 (ENTPD5) ectonucleoside triphosphate diphosphohydrolase gene. Int J Oncol. 19(6): 1249-54.

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Keywords

ENTPD5 Protein, Human, Recombinant (His) NTPDase-5 ectonucleoside triphosphate diphosphohydrolase 5 CD39L4 PCPH recombinant recombinant-proteins proteins protein

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