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Enterokinase Protein, Bovine, Recombinant (His)

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货号 TMPS-00025

别名 PRSS7, ENTK, Enteropeptidase

Enterokinase (EK) is a duodenal protease involved in digestion by activating trypsinogen into trypsin, which in turn activates other pancreatic enzymes. EK specifically cleaves after the sequence Asp-Asp-Asp-Asp-Lys (DDDDK↓) and is inactive if a proline follows the site.
Recombinant bovine enterokinase (rbEK) shows higher activity than EK from other species and is widely used in biochemical applications. It is a glycosylated single-chain protein of ~200 amino acids, tagged with C-terminal 6×His for Ni²⁺ affinity purification and easy removal after digestion. The purified rbEK has a molecular weight of ~40 kDa and is fully biologically active.

Enterokinase Protein, Bovine, Recombinant (His)
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Enterokinase Protein, Bovine, Recombinant (His)

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Rating icon 还可以
货号 TMPS-00025 别名 PRSS7, ENTK, Enteropeptidase

Enterokinase (EK) is a duodenal protease involved in digestion by activating trypsinogen into trypsin, which in turn activates other pancreatic enzymes. EK specifically cleaves after the sequence Asp-Asp-Asp-Asp-Lys (DDDDK↓) and is inactive if a proline follows the site.
Recombinant bovine enterokinase (rbEK) shows higher activity than EK from other species and is widely used in biochemical applications. It is a glycosylated single-chain protein of ~200 amino acids, tagged with C-terminal 6×His for Ni²⁺ affinity purification and easy removal after digestion. The purified rbEK has a molecular weight of ~40 kDa and is fully biologically active.

规格价格库存数量
5 μg
¥ 528
6-8日内发货
10 μg
¥ 863
6-8日内发货
20 μg
¥ 987
5日内发货
50 μg
¥ 1,290
5日内发货
100 μg
¥ 1,980
5日内发货
200 μg
¥ 3,250
5日内发货
500 μg
¥ 5,930
5日内发货
1 mg
¥ 9,720
5日内发货
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TargetMol的所有产品仅用作科学研究或药证申报，不能被用于人体，我们不向个人提供产品和服务。请您遵守承诺用途，不得违反法律法规规定用于任何其他用途。
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重组蛋白用户指南

产品介绍

生物活性
产品描述
Enterokinase (EK) is a duodenal protease involved in digestion by activating trypsinogen into trypsin, which in turn activates other pancreatic enzymes. EK specifically cleaves after the sequence Asp-Asp-Asp-Asp-Lys (DDDDK↓) and is inactive if a proline follows the site.
Recombinant bovine enterokinase (rbEK) shows higher activity than EK from other species and is widely used in biochemical applications. It is a glycosylated single-chain protein of ~200 amino acids, tagged with C-terminal 6×His for Ni²⁺ affinity purification and easy removal after digestion. The purified rbEK has a molecular weight of ~40 kDa and is fully biologically active.
生物活性
100 IU/μg
研究背景
Enterokinase (EK) is a duodenal protease involved in digestion by activating trypsinogen into trypsin, which in turn activates other pancreatic enzymes. EK specifically cleaves after the sequence Asp-Asp-Asp-Asp-Lys (DDDDK↓) and is inactive if a proline follows the site.
种属
Bovine
标签His
蛋白纯度
> 95% as determined by SDS-PAGE.
缓冲液Lyophilized from a 0.2 μm filtered solution in 20mM Tris-HCl, pH 7.4, 200mM NaCl, 2mM CaCl2.
复溶方法Reconstitute lyophilized EK powder in sterile deionized water at 1mg/ml, then dilute in EK Dilution/Storage Buffer according to your requirement.
别名PRSS7, ENTK, Enteropeptidase
内毒素<0.2 EU/μg, determined by LAL method.
化学信息
分子量40 kDa (Reducing conditions)
储存&溶解度
运输方式In general, Lyophilized powders are shipping with blue ice. Solutions are shipping with dry ice.
存储Lyophilized recombinant Bovine Enterokinase (His-tagged) (rbEK) remains stable up to one year at -20°C from date of receipt. Please avoid freeze-thaw cycles.

计算器

  • 复溶 计算器
  • 重组蛋白稀释 计算器
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剂量转换

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技术支持

请阅读 重组蛋白用户指南 了解更多具体信息.
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