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EGFR Protein, Human, Recombinant (His) V2

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货号 TMPY-00742U

别名 PIG61, NISBD2, mENA, HER1, ERBB1, ERBB, epidermal growth factor receptor

EGFR Protein, Human, Recombinant (His) V2 is expressed in HEK293 Cells. The accession number is P00533-1.

EGFR Protein, Human, Recombinant (His) V2
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EGFR Protein, Human, Recombinant (His) V2

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货号 TMPY-00742U 别名 PIG61, NISBD2, mENA, HER1, ERBB1, ERBB, epidermal growth factor receptor

EGFR Protein, Human, Recombinant (His) V2 is expressed in HEK293 Cells. The accession number is P00533-1.

规格价格库存数量
5 μg
¥ 493
现货
10 μg
¥ 788
现货
20 μg
¥ 1,330
现货
50 μg
¥ 2,630
现货
100 μg
¥ 4,450
现货
200 μg
¥ 7,970
5日内发货
500 μg
¥ 17,300
5日内发货
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纯度: > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
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产品介绍


生物活性
产品描述
EGFR Protein, Human, Recombinant (His) V2 is expressed in HEK293 Cells. The accession number is P00533-1.
生物活性
Immobilized Human EGFR, His Tag at 2 μg/ml (100 μl/well) on the plate. Dose response curve for Human EGF, hFc Tag with the EC50 of 15.6 ng/ml determined by ELISA. (QC Test) Loaded Human EGF, hFc Tag on ProA-Biosensor can bind Human EGFR, His Tag with an affinity constant of 0.25 µM as determined in BLI assay (Gator® Prime). Loaded Human TGF-alpha, hFc Tag on ProA-Biosensor can bind Human EGFR, His Tag with an affinity constant of 0.27 µM as determined in BLI assay (Gator® Prime).
研究背景
The epidermal growth factor receptor is a transmembrane protein that is a receptor for members of the epidermal growth factor family of extracellular protein ligands. The epidermal growth factor receptor is a member of the ErbB family of receptors, a subfamily of four closely related receptor tyrosine kinases: EGFR, HER2/neu, Her 3 and Her 4. Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses.
种属
Human
表达系统
HEK293 Cells
标签C-His
蛋白编号P00533-1
蛋白构建Leu25-Ser645
蛋白纯度
> 95% as determined by Bis-Tris PAGE; > 95% as determined by HPLC
蛋白性状Lyophilized powder
缓冲液Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
复溶方法Reconstitute the lyophilized protein in sterile deionized water. The product concentration should not be less than 100 μg/mL. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
别名PIG61, NISBD2, mENA, HER1, ERBB1, ERBB, epidermal growth factor receptor
内毒素< 0.1 EU/μg of the protein as determined by the LAL method.
化学信息
分子量70.19 kDa (Predicted); 80-110 kDa (Due to glycosylation)
颜色White
物理性状Lyophilized powder
储存&溶解度
运输方式In general, Lyophilized powders are shipping with blue ice. Solutions are shipping with dry ice.
存储Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

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