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EGFR Protein, Human, Recombinant (His) V2 is expressed in HEK293 Cells. The accession number is P00533-1.

| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 5 μg | ¥ 493 | 现货 | |
| 10 μg | ¥ 788 | 现货 | |
| 20 μg | ¥ 1,330 | 现货 | |
| 50 μg | ¥ 2,630 | 现货 | |
| 100 μg | ¥ 4,450 | 现货 | |
| 200 μg | ¥ 7,970 | 5日内发货 | |
| 500 μg | ¥ 17,300 | 5日内发货 |
| 生物活性 | Immobilized Human EGFR, His Tag at 2 μg/ml (100 μl/well) on the plate. Dose response curve for Human EGF, hFc Tag with the EC50 of 15.6 ng/ml determined by ELISA. (QC Test)
Loaded Human EGF, hFc Tag on ProA-Biosensor can bind Human EGFR, His Tag with an affinity constant of 0.25 µM as determined in BLI assay (Gator® Prime).
Loaded Human TGF-alpha, hFc Tag on ProA-Biosensor can bind Human EGFR, His Tag with an affinity constant of 0.27 µM as determined in BLI assay (Gator® Prime). |
| 产品描述 | EGFR Protein, Human, Recombinant (His) V2 is expressed in HEK293 Cells. The accession number is P00533-1. |
| 种属 | Human |
| 表达系统 | HEK293 Cells |
| 标签 | C-His |
| 蛋白编号 | P00533-1 |
| 别名 | PIG61,NISBD2,mENA,HER1,ERBB1,ERBB,epidermal growth factor receptor |
| 蛋白构建 | Leu25-Ser645 |
| 蛋白纯度 | > 95% as determined by Bis-Tris PAGE;
> 95% as determined by HPLC |
| 分子量 | 70.19 kDa (Predicted); 80-110 kDa (Due to glycosylation) |
| 内毒素 | < 0.1 EU/μg of the protein as determined by the LAL method. |
| 蛋白性状 | Lyophilized powder |
| 缓冲液 | Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| 复溶方法 | Reconstitute the lyophilized protein in sterile deionized water. The product concentration should not be less than 100 μg/mL. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing. |
| 存储 | Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
| 运输方式 | In general, Lyophilized powders are shipping with blue ice. Solutions are shipping with dry ice. |
| 研究背景 | The epidermal growth factor receptor is a transmembrane protein that is a receptor for members of the epidermal growth factor family of extracellular protein ligands. The epidermal growth factor receptor is a member of the ErbB family of receptors, a subfamily of four closely related receptor tyrosine kinases: EGFR, HER2/neu, Her 3 and Her 4. Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. |