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EGF Protein, Mouse, Recombinant

EGF Protein, Mouse, Recombinant
产品编号 TMPY-03701

EGF Protein, Mouse, Recombinant is expressed in HEK293 mammalian cells. The predicted molecular weight is 6.2 kDa; 14 kDa, reducing conditions and the accession number is P01132.

EGF Protein, Mouse, Recombinant

EGF Protein, Mouse, Recombinant

EGF Protein, Mouse, Recombinant
产品编号 TMPY-03701
EGF Protein, Mouse, Recombinant is expressed in HEK293 mammalian cells. The predicted molecular weight is 6.2 kDa; 14 kDa, reducing conditions and the accession number is P01132.
规格价格库存数量
100 μg
¥ 564
现货
500 μg
¥ 1,059
5日内发货
1 mg
¥ 1,752
5日内发货
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实验操作小课堂
EGF Protein, Mouse, Recombinant产品问题解答
请问 EGF Protein, Mouse, Recombinant 因子是鼠源的 EGF 还是人源的?
EGF Protein, Mouse, Recombinant 重组蛋白是鼠源的,但重组蛋白表达的这段序列 ,鼠和人的序列同源性比较高,有种属交叉性,所以它也有用在人源类器官上。
常见问题解答
化合物带有盐酸盐离子、硫酸盐离子等是否和其本身有什么区别?盐形式和游离态有什么区别?
盐和非盐形式化合物的活性分子是一样的,在生物实验中起到的效果也一致,活性和使用方法都是一样的。只是由于呈盐不同,物理性质比如溶解度会有差异。建议您根据溶解、实验需求进行选择。
如何选择某个靶点的特异性或总的抑制剂?特异性和非特异性的区别是什么?
抑制剂按照特异性分为广谱 pan 和特异性 selective 两种。Pan 为某个靶点总的抑制剂,对所有亚型或整个家族的成员都有抑制作用。Selective 抑制剂针对某个蛋白激酶的某个亚型或家族中某个成员抑制率特别高或有特异性抑制作用。 一般评价一个抑制剂的抑制效率主要看 IC50 值,IC50 值越低,说明抑制剂效率越高。建议您根据以上几个特征进行综合选择,也可联系技术帮您推荐相关抑制剂。
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产品信息

生物活性
1.Measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblast cells. The ED50 for this effect is typically 0.1-0.6 ng/mL.
2.Human intestinal cancer organoids were cultured with RSPO1 (Cat#TMPY-03626), EGF (Cat#TMPY-03701), NOG (Cat#TMPY-02594).
3.Human colorectum organoids were cultured with RSPO1 (Cat#TMPY-03626), EGF (Cat# TMPY-03701), NOG (Cat#TMPY-02594).
4.Human lung cancer organoids were cultured with FGF2 (Cat#TMPY-00749), FGF4 (Cat#TMPY-05004), FGF7 (Cat#TMPY-00403), EGF (Cat#TMPY-03701), FGF10 (Cat#TMPY-01061), NOG (Cat#TMPY-02594), RSPO1(Cat#TMPY-03626).
5.Human lung organoids were cultured with FGF2 (Cat#TMPY-00749), FGF4 (Cat#TMPY-05004), FGF7 (Cat#TMPY-00403), EGF (Cat#TMPY-03701), FGF10 (Cat#TMPY-01061), NOG (Cat#TMPY-02594), RSPO1 (Cat#TMPY-03626).
6.Human cholangiocarcinomas organoids were cultured with FGF2 (Cat#TMPY-00749), HGF (Cat#TMPY-02327), FGF7 (Cat#TMPY-00403), EGF (Cat#TMPY-03701), FGF10 (Cat#TMPY-01061), NOG (Cat#TMPY-02594), RSPO1 (Cat#TMPY-03626).
7.Human liver cancer organoids were cultured with FGF2 (Cat#TMPY-00749), HGF (Cat#TMPY-02327), FGF7 (Cat#TMPY-00403), EGF (Cat#TMPY-03701), FGF10 (Cat#TMPY-01061), TGFB1 (Cat#TMPY-02638), NOG (Cat#TMPY-02594), RSPO1 (Cat#TMPY-03626). 8.Human kidney cancer organoids were cultured with FGF2 (Cat#TMPY-00749), FGF7 (Cat#TMPY-00403), EGF (Cat#TMPY-03701), FGF10 (Cat#TMPY-01061), NOG (Cat#TMPY-02594), RSPO1 (Cat#TMPY-03626).
9.Human kidney organoids were cultured with FGF7 (Cat#TMPY-00403), EGF (Cat#TMPY-03701), FGF10 (Cat#TMPY-01061), NOG (Cat#TMPY-02594), RSPO1 (Cat#TMPY-03626), HGF (Cat#TMPY-02327), FGF4 (Cat#TMPY-05004).
10.Human gastric cancer organoids were cultured with EGF (Cat#TMPY-03701), FGF10 (Cat#TMPY-01061), NOG (Cat#TMPY-02594), RSPO1 (Cat#TMPY-03626).
11.Human stomach organoids organoids were cultured with EGF (Cat#TMPY-03701), FGF10 (Cat#TMPY-01061), NOG (Cat#TMPY-02594), RSPO1 (Cat#TMPY-03626).
12.Human breast cancer organoids were cultured with FGF7 (Cat#TMPY-00403), RSPO1 (Cat#TMPY-03626), IGF1 (Cat#TMPY-06982), EGF (Cat#TMPY-03701), NRG1 Beta 1 (Cat#TMPY-02600), NOG (Cat#TMPY-02594).
13.Human ovarian organoids were cultured with IGF1 (Cat#TMPY-06982), NRG1 Beta 1 (Cat#TMPY-02600), RSPO1 (Cat#TMPY-03626), EGF (Cat#TMPY-03701), NOG (Cat#TMPY-02594).
14.Human small intestinal organoids were cultured with IL22 (Cat#TMPY-03490), FGF10 (Cat#TMPY-01061), EGF (Cat#TMPY-03701), NOG (Cat#TMPY-02594).
产品描述
EGF Protein, Mouse, Recombinant is expressed in HEK293 mammalian cells. The predicted molecular weight is 6.2 kDa; 14 kDa, reducing conditions and the accession number is P01132.
种属
Mouse
表达系统
HEK293 Cells
标签Tag Free
蛋白编号P01132
蛋白构建
A DNA sequence encoding the mouse EGF (P01132) (Asn977-Arg1029) was expressed and purified with two additional amino acids (Gly & Pro) at the N-terminus.
蛋白纯度
> 95 % as determined by SDS-PAGE
分子量6.2 kDa (predicted); 14 kDa (reducing conditions)
内毒素< 1.0 EU/μg of the protein as determined by the LAL method.
缓冲液Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. . Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
复溶方法
A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
存储
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
运输方式In general, Lyophilized powders are shipping with blue ice.

计算器

  • 复溶 计算器
  • 重组蛋白稀释 计算器
  • 比活力 计算器

技术支持

请阅读 重组蛋白用户指南 了解更多具体信息.