DNA helicase II Protein, E. coli, Recombinant

A helicase with DNA-dependent ATPase activity. Unwinds DNA duplexes with 3' to 5' polarity with respect to the bound strand. Initiates unwinding more efficiently from a nicked substrate than ds duplex DNA. Involved in the post-incision events of nucleotide excision repair and methyl-directed mismatch repair, and probably also in repair of alkylated DNA. DNA helicase II Protein, E. coli, Recombinant is expressed in E. coli expression system. The predicted molecular weight is 82.0 kDa and the accession number is P03018.

规格	价格/CNY		数量
20 μg	￥ 2,790	20日内发货
100 μg	￥ 4,400	20日内发货
1 mg	￥ 15,600	20日内发货

规格

价格/CNY

货期

数量

20 μg

￥ 2,790

20日内发货

100 μg

￥ 4,400

20日内发货

1 mg

￥ 15,600

20日内发货

产品描述

种属	E. coli
表达系统	E. coli
标签	Tag Free
蛋白编号	P03018
别名	mutU, DNA 3'-5' helicase II, DNA helicase II, recL, rad, pdeB, uvrD
氨基酸序列	MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIAWLMSVENCSPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHHMDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQSYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNILVDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAETIRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVVNRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDALSYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACRELLQEKALAGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIENLEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTLHSAKGLEFPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKEVYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFGEGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLESV
蛋白构建	1-720 aa
蛋白纯度	> 85% as determined by SDS-PAGE.
分子量	82.0 kDa (predicted)

缓冲液	Tris-based buffer, 50% glycerol
复溶方法	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
存储	Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted protein solutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
运输方式	In general, Lyophilized powders are shipping with blue ice. Solutions are shipping with dry ice.
研究背景	A helicase with DNA-dependent ATPase activity. Unwinds DNA duplexes with 3' to 5' polarity with respect to the bound strand. Initiates unwinding more efficiently from a nicked substrate than ds duplex DNA. Involved in the post-incision events of nucleotide excision repair and methyl-directed mismatch repair, and probably also in repair of alkylated DNA.

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复溶计算器

重组蛋白稀释计算器

比活力计算器

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

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DNA helicase II Protein, E. coli, Recombinant

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技术支持

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