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DNA helicase II Protein, E. coli, Recombinant
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货号 TMPH-00611
别名 uvrD, recL, rad, pdeB, mutU, DNA helicase II, DNA 3'-5' helicase II
A helicase with DNA-dependent ATPase activity. Unwinds DNA duplexes with 3' to 5' polarity with respect to the bound strand. Initiates unwinding more efficiently from a nicked substrate than ds duplex DNA. Involved in the post-incision events of nucleotide excision repair and methyl-directed mismatch repair, and probably also in repair of alkylated DNA. DNA helicase II Protein, E. coli, Recombinant is expressed in E. coli expression system. The predicted molecular weight is 82.0 kDa and the accession number is P03018.
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DNA helicase II Protein, E. coli, Recombinant
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货号 TMPH-00611 别名 uvrD, recL, rad, pdeB, mutU, DNA helicase II, DNA 3'-5' helicase II
A helicase with DNA-dependent ATPase activity. Unwinds DNA duplexes with 3' to 5' polarity with respect to the bound strand. Initiates unwinding more efficiently from a nicked substrate than ds duplex DNA. Involved in the post-incision events of nucleotide excision repair and methyl-directed mismatch repair, and probably also in repair of alkylated DNA. DNA helicase II Protein, E. coli, Recombinant is expressed in E. coli expression system. The predicted molecular weight is 82.0 kDa and the accession number is P03018.
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 5 μg | ¥ 998 | 20日内发货 | |
| 10 μg | ¥ 1,660 | 20日内发货 | |
| 20 μg | ¥ 2,790 | 20日内发货 | |
| 50 μg | ¥ 3,790 | 20日内发货 | |
| 100 μg | ¥ 4,830 | 20日内发货 | |
| 200 μg | ¥ 6,880 | 20日内发货 | |
| 500 μg | ¥ 11,500 | 20日内发货 | |
| 1 mg | ¥ 16,800 | 20日内发货 |
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| 产品描述 | A helicase with DNA-dependent ATPase activity. Unwinds DNA duplexes with 3' to 5' polarity with respect to the bound strand. Initiates unwinding more efficiently from a nicked substrate than ds duplex DNA. Involved in the post-incision events of nucleotide excision repair and methyl-directed mismatch repair, and probably also in repair of alkylated DNA. DNA helicase II Protein, E. coli, Recombinant is expressed in E. coli expression system. The predicted molecular weight is 82.0 kDa and the accession number is P03018. |
| 生物活性 | Activity has not been tested. It is theoretically active, but we cannot guarantee it. If you require protein activity, we recommend choosing the eukaryotic expression version first. |
| 研究背景 | A helicase with DNA-dependent ATPase activity. Unwinds DNA duplexes with 3' to 5' polarity with respect to the bound strand. Initiates unwinding more efficiently from a nicked substrate than ds duplex DNA. Involved in the post-incision events of nucleotide excision repair and methyl-directed mismatch repair, and probably also in repair of alkylated DNA. |
| 种属 | E. coli |
| 表达系统 | E. coli |
| 标签 | Tag Free |
| 蛋白编号 | P03018 |
| 氨基酸序列 | MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIAWLMSVENCSPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHHMDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQSYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNILVDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAETIRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVVNRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDALSYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACRELLQEKALAGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIENLEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTLHSAKGLEFPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKEVYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFGEGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLESV |
| 蛋白构建 | 1-720 aa |
| 蛋白纯度 | > 85% as determined by SDS-PAGE. |
| 缓冲液 | Tris-based buffer, 50% glycerol |
| 复溶方法 | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
| 别名 | uvrD, recL, rad, pdeB, mutU, DNA helicase II, DNA 3'-5' helicase II |
| 内毒素 | < 1.0 EU/μg of the protein as determined by the LAL method. |
| 分子量 | 82.0 kDa (predicted) |
| 运输方式 | In general, Lyophilized powders are shipping with blue ice. Solutions are shipping with dry ice. |
| 存储 | Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
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