DDR2 重组蛋白, Rat (hFc)

DDR2 Protein, Rat, Recombinant (hFc)

产品编号 TMPY-03098

DDR2 Protein, Rat, Recombinant (hFc) is expressed in HEK293 mammalian cells with hFc tag. The predicted molecular weight is 69.5 kDa and the accession number is B1WC09.

规格	价格	库存	数量
100 μg	¥ 3,820	5日内发货

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常见问题解答

问为什么重组蛋白的体积和质量与预期不符？

答蛋白冻干粉由蛋白原液冻干而来，冻干粉质量不等同于蛋白质量。由于冻⼲产品的体积大⼩受多种因素影响，包括冻⼲前缓冲液的组分、盐离⼦浓度以及产品本身的浓度等。冻⼲粉可能看上去很多，也可能肉眼不易观察到，这些都是正常现象，请您放心使用。在收到产品后，照常复溶、使用或分装储存即可。

问蛋白能保证活性吗？

答我们有现货的产品，有活性数据的话会展示在官网生物活性那栏，没有数据的也不代表就一定没有活性，是我们还没有建立活性检测方法，没有进行测试，但是我们的蛋白都是优先按照有活性的方法去表达纯化的。

问重组蛋白产品是如何运输的？

答大部分重组蛋白产品为冻⼲粉，对温度不敏感，在室温下稳定，一般室温或蓝冰运输。对于液体类蛋白，一般采用⼲冰运输。如果您收到产品时发现冰盒已经融化，无需担心，蛋白可以正常使用，短时间内不会影响产品质量。如果特定蛋白在室温下不稳定，我们会提供相应的低温运输方式，以确保产品质量不受影响。

问为什么某些重组蛋白溶液中还需要添加载体蛋白？

答载体蛋白通常被用于提高蛋白的稳定性，防止蛋白在冷冻或解冻过程中粘附在管壁上。塑料管对蛋白有一定的吸附能力，这可能导致蛋白和管壁之间难以分离，导致溶液中蛋白的实际浓度下降，从而影响其活性。为减少这种损失，在长期保存重组蛋白产品之前，建议添加常用的载体蛋白溶液，例如0.1% BSA（牛血清白蛋白）、5% HSA（人血清白蛋白）、10% FBS（胎牛血清）或 5% 海藻糖等。如果重组蛋白的浓度较低，强烈建议添加适量的载体蛋白。

问重组蛋白有吗？

答您需要什么种属的蛋白呢，对表达系统和标签有要求吗，用于什么实验？

产品信息

生物活性	Measured by its ability to bind with Rat tail Collagen I in a functional ELISA.
产品描述	DDR2 Protein, Rat, Recombinant (hFc) is expressed in HEK293 mammalian cells with hFc tag. The predicted molecular weight is 69.5 kDa and the accession number is B1WC09.
种属	Rat
表达系统	HEK293 Cells
标签	C-hFc
蛋白编号	B1WC09
别名	discoidin domain receptor tyrosine kinase 2
蛋白构建	A DNA sequence encoding the rat DDR2 (B1WC09) (Met1-Arg399) was expressed, fused with the Fc region of human IgG1 at the C-terminus. Predicted N terminal: Gln 24
蛋白纯度	> 80 % as determined by SDS-PAGE
分子量	69.5 kDa (predicted); 110 kDa (reducing conditions)
内毒素	< 1.0 EU/μg of the protein as determined by the LAL method.
缓冲液	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
复溶方法	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
存储	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
运输方式	In general, Lyophilized powders are shipping with blue ice.
研究背景	Discoidin domain receptor 2 (DDR2) or CD167b (cluster of differentiation 167b) is a kind of protein tyrosine kinases associated with cell proliferation and tumor metastasis, and collagen, identified as a ligand for DDR2, up-regulates matrix metallloproteinase 1 (MMP-1) and MMP-2 expression in cellular matrix. DDR2/CD167b was found to recognise the triple-helical region of collagen X as well as the NC1 domain. Binding to the collagenous region was dependent on the triple-helical conformation. DDR2/CD167b autophosphorylation was induced by the collagen X triple-helical region but not the NC1 domain, indicating that the triple-helical region of collagen X contains a specific DDR2 binding site that is capable of receptor activation. DDR2/CD167b is induced during stellate cell activation and implicate the phosphorylated receptor as a mediator of MMP-2 release and growth stimulation in response to type I collagen. Moreover, type I collagen-dependent upregulation of DDR2/CD167b expression establishes a positive feedback loop in activated stellate cells, leading to further proliferation and enhanced invasive activity.Cancer ImmunotherapyImmune CheckpointImmunotherapyTargeted Therapy

DDR2 Protein, Rat, Recombinant (hFc)

DDR2 Protein, Rat, Recombinant (hFc)

产品信息

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