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CSF1R Protein, Human, Recombinant

产品编号 TMPY-00881
CSF1R Protein, Human, Recombinant is expressed in HEK293 mammalian cells. The predicted molecular weight is 55.1 kDa and the accession number is P07333-1.
规格价格库存数量
200 μg
¥ 3,820
5日内发货
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产品信息

生物活性
1. Immobilized sMCSFR at 10 μg/ml (100 μl/well) can bind biotinylated human CSF1-His , The EC50 of biotinylated human CSF1-His is 32-74.8 ng/ml. 2. Measured by its ability to inhibit the human CSF-induced proliferation of M‑NFS‑60 mouse myelogenous leukemia lymphoblast cells. The ED50 for this effect is typically 0.3-1.5 μg/mL in the presence of 3 ng/mL of rhM-CSF.
产品描述
CSF1R Protein, Human, Recombinant is expressed in HEK293 mammalian cells. The predicted molecular weight is 55.1 kDa and the accession number is P07333-1.
种属
Human
表达系统
HEK293 Cells
标签Tag Free
蛋白编号P07333-1
别名
M-CSF-R,MCSF Receptor,HDLS,FMS,FIM2,CSFR,CSF-1R,colony stimulating factor 1 receptor,C-FMS,CD115
蛋白构建
The mature form of human M-CSFR (NP_005202.2) extracellular domain (Met 1-Glu 512) with quinary-aa peptide (DDDDK) at the C-terminus was expressed and purified. Predicted N terminal: Ile 20
蛋白纯度
> 90 % as determined by SDS-PAGE
分子量55.1 kDa (predicted); 80-90 kDa (reducing condition, due to glycosylation)
内毒素< 1.0 EU/μg of the protein as determined by the LAL method.
缓冲液Lyophilized from a solution filtered through a 0.22 μm filter, containing 100 mM Glycine, 10 mM NaCl, 50 mM Tris, pH 7.5. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
复溶方法
A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
存储
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
运输方式In general, Lyophilized powders are shipping with blue ice.
研究背景
M-CSFR encoded by the proto-oncogene c-fms is the receptor for colony stimulating factor 1 (CSF1R), a cytokine involved in the proliferation, differentiation, and activation of macrophages. This cell surface glycoprotein is consisted by an extracellular ligand-binding domain, a single membrane-spanning segment, and an intracellular tyrosine kinase domain. Binding of CSF1 activates the receptor kinase, leading to "autophosphorylation" of receptor subunits and the concomitant phosphorylation of a series of cellular proteins on tyrosine residues. CSF1R is a tyrosine kinase receptor that is absolutely required for macrophage differentiation and thus occupies a central role in hematopoiesis. CSF1 and its receptor (CSF1R, product of c-fms proto-oncogene) were initially implicated as essential for normal monocyte development as well as for trophoblastic implantation. This apparent role for CSF1/CSF1R in normal mammary gland development is very intriguing because this receptor/ligand pair has also been found to be important in the biology of breast cancer in which abnormal expression of CSF1 and its receptor correlates with tumor cell invasiveness and adverse clinical prognosis. Tumor cell expression of CSF1R is under the control of several steroid hormones (glucocorticoids and progestins) and the binding of several bHLH transcription factors, while tumor cell expression of CSF-1 appears to be regulated by other hormones, some of which are involved in normal lactogenic differentiation. However, studies have demonstrated that CSF1 and CSF1R have additional roles in mammary gland development during pregnancy and lactation. The role of CSF1 and CSF1R in normal and neoplastic mammary development that may elucidate potential relationships of growth factor-induced biological changes in the breast during pregnancy and tumor progression.

参考文献

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  • 复溶 计算器
  • 重组蛋白稀释 计算器
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