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Coagulation factor IX/F9 Protein, Mouse, Recombinant (His)

产品编号 TMPY-07195

Coagulation factor IX/F9 Protein, Mouse, Recombinant (His) is expressed in HEK293 Cells. The accession number is P16294.

Coagulation factor IX/F9 Protein, Mouse, Recombinant (His)

Coagulation factor IX/F9 Protein, Mouse, Recombinant (His)

产品编号 TMPY-07195
Coagulation factor IX/F9 Protein, Mouse, Recombinant (His) is expressed in HEK293 Cells. The accession number is P16294.
规格价格库存数量
20 μg
¥ 2,270
期货
大包装 & 定制
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常见问题解答
产品是否需要避光?
一般来说,如果有需要避光储存的分子,我们会选择棕色玻璃瓶发货的。
Tween-20 可以用 Tween-80 替代吗?
不建议使用 Tween-20。相较于 Tween-20,Tween-80 的耐受性更好,Tween-80已被用作评估实验药物和毒物的行为影响的工具,且没有明显的副作用。 参考文献:Castro CA, Hogan JB, Benson KA, Shehata CW, Landauer MR. Behavioral effects of vehicles: DMSO, ethanol, Tween-20, Tween-80, and emulphor-620. Pharmacol Biochem Behav. 1995 Apr;50(4):521-6.
超声一般需要多久?
超声时长和溶解的量和浓度都有关,一般建议半个小时左右,如果浓度接近最大溶解度,或者需溶解的粉末较多,建议延长超声时间,并辅以加热。
收到粉状试剂粘壁严重,如何处理?
建议您用离心机 3000 rpm下离心一会儿,将粉末收集到管底。
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产品信息

生物活性
Activity testing is in progress. It is theoretically active, but we cannot guarantee it. If you require protein activity, we recommend choosing the eukaryotic expression version first.
产品描述
Coagulation factor IX/F9 Protein, Mouse, Recombinant (His) is expressed in HEK293 Cells. The accession number is P16294.
种属
Mouse
表达系统
HEK293 Cells
标签C-His
蛋白编号P16294
别名
Cf-9,Cf9
蛋白构建
A DNA sequence encoding the full length of mouse F9 (P16294) (Met 1-Thr 471) was fused with a polyhistidine tag at the C-terminus and a signal peptide at the N-terminus.
蛋白纯度
≥ 95% as determined by SDS-PAGE. ≥ 95% as determined by SEC-HPLC.
分子量51.3 kDa (predicted); 60-80 kDa (reducing contition, due to glycosylation)
内毒素< 1.0 EU per μg of the protein as determined by the LAL method
缓冲液Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
复溶方法
Please refer to the lot-specific COA.
存储
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
运输方式In general, Lyophilized powders are shipping with blue ice.
研究背景
Coagulation factor IX, also known as Christmas factor, Plasma thromboplastin component and PTC, is a secreted protein which belongs to the peptidase S1 family. Coagulation factor IX / F9 contains two EGF-like domains, one Gla (gamma-carboxy-glutamate) domain and one?peptidase S1 domain. Coagulation factor IX / F9 is a vitamin K-dependent plasma protein that participates in the intrinsic pathway of blood coagulation by converting factor X to its active form in the presence of Ca2+ons, phospholipids, and factor VIIIa. Defects in Coagulation factor IX / F9 are the cause of thrombophilia due to factor IX defect which is a hemostatic disorder characterized by a tendency to thrombosis. Defects in Coagulation factor IX / F9 are also the cause of recessive X-linked hemophilia B ( HEMB ) which also known as Christmas disease.

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