CLEC3A Protein, Mouse, Recombinant (His)

产品编号 TMPY-05250

C-type lectin domain family 3 member A (CLEC3A) is a poorly characterized protein belonging to the superfamily of C-type lectins. Elevated CLEC3A expression may correlate with breast IDC metastatic potential and indicated a poor prognosis in breast IDC. CLEC3A knockdown inhibited BC cell growth and metastasis might be through suppressing PI3K/AKT signaling activity. That CLEC3A is a promising therapeutic target for BC in the future. Matrilysin (MMP-7) plays important roles in tumor progression. Previous studies have suggested that MMP-7 binds to tumor cell surface and promotes their metastatic potential. C-type lectin domain family 3 member A (CLEC3A) as a membrane-bound substrate of MMP-7. CLEC3A binds to heparan sulfate proteoglycans on cell surface, leading to the enhancement of cell adhesion to integrin ligands on ECM. It can be speculated that the cleavage of CLEC3A by MMP-7 weakens the stable adhesion of tumor cells to the matrix and promotes their migration in tumor microenvironments.

CLEC3A Protein, Mouse, Recombinant (His)

产品编号 TMPY-05250

规格	价格	库存	数量
20 μg	¥ 1,890	5日内发货
100 μg	¥ 4,460	5日内发货

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常见问题解答

问超声应使用什么仪器？

答建议使用“超声清洗仪”，不推荐用“超声破碎仪”。

问如何设置母液浓度？

答母液浓度需低于官网给出的溶解度，在这个范围内，根据工作液浓度设置母液浓度，细胞实验中，建议母液浓度设定在工作液浓度的 1000 倍以上。

问收到化合物后，是否需要称量后配制储备液？

答对于小包装，如 1 mg、5 mg、10 mg 等，不建议二次称量。这样会导致化合物损耗，且无法估算。TargetMol 所有产品原装进口，精准称量，您收到后可直接添加相应量溶剂。对于大包装，如果您需要分批次实验，可每次称量后配制储备液，建议您 10 mg 起称，称量的误差会小一些；当然条件允许，也可以先配制成高浓度母液，分装备用。

问收到产品发现冰盒都化了，怎么办？

答一般化合物粉末对温度不敏感，加冰盒是为了防止极端气温，如果冰盒融化不影响产品质量。

问Tween-20 可以用 Tween-80 替代吗？

答不建议使用 Tween-20。相较于 Tween-20，Tween-80 的耐受性更好，Tween-80已被用作评估实验药物和毒物的行为影响的工具，且没有明显的副作用。参考文献：Castro CA， Hogan JB， Benson KA， Shehata CW， Landauer MR. Behavioral effects of vehicles: DMSO， ethanol， Tween-20， Tween-80， and emulphor-620. Pharmacol Biochem Behav. 1995 Apr;50(4):521-6.

产品信息

生物活性	Activity testing is in progress. It is theoretically active, but we cannot guarantee it. If you require protein activity, we recommend choosing the eukaryotic expression version first.
产品描述	C-type lectin domain family 3 member A (CLEC3A) is a poorly characterized protein belonging to the superfamily of C-type lectins. Elevated CLEC3A expression may correlate with breast IDC metastatic potential and indicated a poor prognosis in breast IDC. CLEC3A knockdown inhibited BC cell growth and metastasis might be through suppressing PI3K/AKT signaling activity. That CLEC3A is a promising therapeutic target for BC in the future. Matrilysin (MMP-7) plays important roles in tumor progression. Previous studies have suggested that MMP-7 binds to tumor cell surface and promotes their metastatic potential. C-type lectin domain family 3 member A (CLEC3A) as a membrane-bound substrate of MMP-7. CLEC3A binds to heparan sulfate proteoglycans on cell surface, leading to the enhancement of cell adhesion to integrin ligands on ECM. It can be speculated that the cleavage of CLEC3A by MMP-7 weakens the stable adhesion of tumor cells to the matrix and promotes their migration in tumor microenvironments.
种属	Mouse
表达系统	Baculovirus Insect Cells
标签	C-His
蛋白编号	B2RVD1
别名	Gm796,C-type lectin domain family 3, member A,Clecsf1,1110019O10Rik
蛋白构建	A DNA sequence encoding the mouse CLEC3A (NP_001007224.1) (Met1-Pro196) was expressed with a polyhistidine tag at the C-terminus. Predicted N terminal: Tyr 23
蛋白纯度	> 90 % as determined by SDS-PAGE.
分子量	21.3 kDa (predicted)
内毒素	< 1.0 EU/μg of the protein as determined by the LAL method.
缓冲液	Lyophilized from a solution filtered through a 0.22 μm filter, containing 20 mM Tris, pH 8.0, 300 mM NaCl, 10% glycerol. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
复溶方法	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
存储	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
运输方式	In general, Lyophilized powders are shipping with blue ice.
研究背景	C-type lectin domain family 3 member A (CLEC3A) is a poorly characterized protein belonging to the superfamily of C-type lectins. Elevated CLEC3A expression may correlate with breast IDC metastatic potential and indicated a poor prognosis in breast IDC. CLEC3A knockdown inhibited BC cell growth and metastasis might be through suppressing PI3K/AKT signaling activity. That CLEC3A is a promising therapeutic target for BC in the future. Matrilysin (MMP-7) plays important roles in tumor progression. Previous studies have suggested that MMP-7 binds to tumor cell surface and promotes their metastatic potential. C-type lectin domain family 3 member A (CLEC3A) as a membrane-bound substrate of MMP-7. CLEC3A binds to heparan sulfate proteoglycans on cell surface, leading to the enhancement of cell adhesion to integrin ligands on ECM. It can be speculated that the cleavage of CLEC3A by MMP-7 weakens the stable adhesion of tumor cells to the matrix and promotes their migration in tumor microenvironments.

CLEC3A Protein, Mouse, Recombinant (His)

CLEC3A Protein, Mouse, Recombinant (His)

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