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CD73/5'-Nucleotidase Protein, Mouse, Recombinant (His)

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产品编号 TMPY-01342

CD73 Protein, Mouse, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 59.4 kDa and the accession number is Q0VEE0.

CD73/5'-Nucleotidase Protein, Mouse, Recombinant (His)

一键复制产品信息

产品编号 TMPY-01342

CD73 Protein, Mouse, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 59.4 kDa and the accession number is Q0VEE0.

规格	价格	库存
5 μg	¥ 429	6-8日内发货
10 μg	¥ 695	6-8日内发货
20 μg	¥ 1,130	5日内发货
50 μg	¥ 2,260	In stock
100 μg	¥ 3,970	5日内发货
200 μg	¥ 6,930	5日内发货
500 μg	¥ 14,300	5日内发货

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产品信息

生物活性	Measured by its ability to hydrolyze the 5’-phosphate group from the substrate adenosine-5’-monophosphate (AMP). The orthophosphate product is measured by a Malachite Green Phosphate Detection Kit . The specific activity is >10,000 pmol/min/µg.
产品描述	CD73 Protein, Mouse, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 59.4 kDa and the accession number is Q0VEE0.
种属	Mouse
表达系统	HEK293 Cells
标签	C-His
蛋白编号	Q61503
别名	Nt5,NT,eNT,CD73,AI447961,5'-nucleotidase, ecto (CD73),2210401F01Rik
蛋白构建	Trp29-Lys549
蛋白纯度	> 95 % as determined by SDS-PAGE
分子量	59.4 kDa (predicted); 59.4 kDa (reducing conditions)
内毒素	< 1.0 EU/μg of the protein as determined by the LAL method.
蛋白性状	Lyophilized powder
缓冲液	1. Lyophilized from 20 mM Tris, 500 mM NaCl, pH 7.4. <br> 2. Lyophilized from 0.22 μm filtered solution in 20 mM Tris, 120 mM NaCl (pH 7.5). <br> Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
复溶方法	Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
存储	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
运输方式	In general, Lyophilized powders are shipping with blue ice.
研究背景	5'-nucleotidase, also known as NT5E, NTE, and CD73, is a cell membrane protein that belongs to the 5'-nucleotidase family. CD73 is a glycosylphosphatidylinositol (GPI) anchored purine salvage enzyme expressed on the surface of human T and B lymphocytes. CD73 catalyzes the conversion of purine and pyrimidine ribo- and deoxyribonucleoside monophosphates to the corresponding nucleosides. CD73 serves as a costimulatory molecule in activating T cells. CD73 generated adenosine functions in cell signaling in many physiologic systems, including intestinal epithelium, ischemic myocardium, and cholinergic synapses. CD73 might mediate lymphocyte-stromal cell interactions or condition the local microenvironment to facilitate lymphocyte development and/or function. In CD73-depleted cells, surface levels of the leukocyte adhesion molecules ICAM-1, VCAM-1, and E-selectin increase. CD73 produces extracellular adenosine, which then acts on G protein-coupled purinergic receptors to induce cellular responses. CD73 has also been reported to regulate the expression of pro-inflammatory molecules in mouse endothelium.