CD40 Ligand Trimer Protein, Human, Recombinant (hFc)

CD40 ligand or CD40L, also called CD154, is a protein that is primarily expressed on activated T cells and is a member of the TNF superfamily of molecules. It binds to CD40 (protein) on antigen-presenting cells (APC), which leads to many effects depending on the target cell type. In total CD40L has three binding partners: CD40, α5β1 integrin and αIIbβ3. CD154 acts as a costimulatory molecule and is particularly important on a subset of T cells called T follicular helper cells.

规格	价格/CNY		数量
100 μg	￥ 2,663	5日内发货
500 μg	￥ 10,682	5日内发货
1 mg	￥ 17,810	5日内发货

规格

价格/CNY

货期

数量

100 μg

￥ 2,663

5日内发货

500 μg

￥ 10,682

5日内发货

1 mg

￥ 17,810

5日内发货

生物活性

1. Immobilized Human CD40, His Tag at 1 μg/ml (100 μl/Well) on the plate. Dose response curve for Human CD40 Ligand (Trimer) , hFc Tag with the EC50 of 9.0 ng/ml determined by ELISA (QC Test).
2. Serial dilutions of Anti-CD40 Antibody were added into Biotinylated Human CD40, His Tag : Human CD40 Ligand (Trimer) , hFc Tag binding reactioins. The half maximal inhibitiory concentration (IC50) is 2.0 μg/ml.

产品描述

种属	Human
表达系统	HEK293 Cells
标签	N-hFc
蛋白编号	P29965
别名	CD40 Ligand, CD40LIGM, IGM, gp39, TNFSF5, TRAP, HIGM1, CD154, TNFSF5IMD3, IMD3, CD40L, T-BAM, CD40LG
蛋白构建	Met113-Leu261
蛋白纯度	> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
分子量	74.9 kDa (predicted). Due to glycosylation, the protein migrates to 75-80 kDa based on Tris-Bis PAGE result.

内毒素	< 1 EU/μg by the LAL method.
缓冲液	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS (pH 7.4). Typically, 8% trehalose is incorporated as a protective agent before lyophilization.
复溶方法	Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
存储	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
运输方式	In general, Lyophilized powders are shipping with blue ice.
研究背景	CD40 ligand or CD40L, also called CD154, is a protein that is primarily expressed on activated T cells and is a member of the TNF superfamily of molecules. It binds to CD40 (protein) on antigen-presenting cells (APC), which leads to many effects depending on the target cell type. In total CD40L has three binding partners: CD40, α5β1 integrin and αIIbβ3. CD154 acts as a costimulatory molecule and is particularly important on a subset of T cells called T follicular helper cells.