生物活性

产品描述	CD3D & CD3E Heterodimer Protein, Human, Recombinant (His & Avi), Biotinylated is expressed in HEK293 Cells. The accession number is P07766(CD3E)&P04234(CD3D).
生物活性	Immobilized Biotinylated Human CD3E&CD3D, His Avi Tag at 0.5 μg/ml (100 μl/well) on the streptavidin precoated plate (5 μg/ml). Dose response curve for OKT3, mFc Tag with the EC50 of 0.11 μg/ml determined by ELISA (QC Test).
研究背景	T-cell surface glycoprotein CD3 epsilon&CD3 delta chain, also known as CD3E&CD3D, are single-pass type I membrane proteins. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain.
种属	Human
表达系统	HEK293 Cells
标签	C-His-Avi
蛋白编号	P07766 (CD3E) & P04234 (CD3D)
蛋白构建	Asp23-Asp126 (CD3E) and Phe22-Ala105 (CD3D)
蛋白纯度	> 95% as determined by Bis-Tris PAGE; > 95% as determined by HPLC
缓冲液	Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
复溶方法	Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
内毒素	< 1 EU/μg of the protein as determined by the LAL method.

化学信息

分子量	17.9 kDa (CD3E) and 18.52 kDa (CD3D) (Predicted); 23-27 kDa and 32-40 kDa (Reducing condition)

储存&溶解度

运输方式

In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.

存储

It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

实际储存温度请以COA为准

CD3D & CD3E Heterodimer Protein, Human, Recombinant (His & Avi), Biotinylated

CD3D & CD3E Heterodimer Protein, Human, Recombinant (His & Avi), Biotinylated

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