生物活性

产品描述	CD3D & CD3E Heterodimer Protein, Cynomolgus, Recombinant (His & Flag) is expressed in HEK293 Cells. The accession number is Q95LI5(CD3E)&Q95LI8(CD3D).
生物活性	Immobilized Cynomolgus CD3E&CD3D, His Tag at 0.5 μg/ml (100 μl/well) on the plate. Dose response curve for Anti-CD3 Antibody, hFc Tag with the EC50 of 7.2 ng/ml determined by ELISA (QC Test).
研究背景	T-cell surface glycoprotein CD3 epsilon&CD3 delta chain, also known as CD3E&CD3D, are single-pass type I membrane proteins. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain.
种属	Cynomolgus
表达系统	HEK293 Cells
标签	C-His, C-Flag
蛋白编号	Q95LI5 (CD3E) & Q95LI8 (CD3D)
蛋白构建	Gln22-Asp117 (CD3E) and Phe22-Ala105 (CD3D)
蛋白纯度	> 95% as determined by Bis-Tris PAGE; > 95% as determined by HPLC
缓冲液	Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
复溶方法	Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
内毒素	< 0.1 EU/μg of the protein as determined by the LAL method.

化学信息

分子量	16.17 kDa (CD3E) and 15.01 kDa (CD3D) (Predicted); 17-23 kDa and 35-45 kDa (Reducing condition)

储存&溶解度

运输方式

In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.

存储

It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

实际储存温度请以COA为准

CD3D & CD3E Heterodimer Protein, Cynomolgus, Recombinant (His & Flag)

CD3D & CD3E Heterodimer Protein, Cynomolgus, Recombinant (His & Flag)

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