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c-Kit/CD117 Protein, Human, Recombinant (His & Avi), Biotinylated
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很棒SPR兼容缓冲液
货号 TMPK-00805
别名 SCFR, PBT, KIT, EC 2.7.10.1, EC 2.7.10, c-kit, ckit, CD117
The c-kit proto-oncogen (CD 117) has been shown to be present in several cell types including normal and neoplastic hemopoietic cells. Among normal BM cells, CD117 expression has been found in about half of the CD34 precursors including progenitors committed to the erythroid, granulo-monocytic, and megakaryocytic cell lineages. CD117 Protein, Human, Recombinant (His & Avi), Biotinylated is expressed in HEK293 mammalian cells with C-His-Avi tag. The predicted molecular weight is 58.60 kDa and the accession number is P10721-2.
其他形式的 “c-Kit/CD117 Protein, Human, Recombinant (His & Avi), Biotinylated”:
c-Kit/CD117 Protein, Human, Recombinant (His & Avi), Biotinylated
一键复制产品信息 很棒SPR兼容缓冲液
货号 TMPK-00805 别名 SCFR, PBT, KIT, EC 2.7.10.1, EC 2.7.10, c-kit, ckit, CD117
The c-kit proto-oncogen (CD 117) has been shown to be present in several cell types including normal and neoplastic hemopoietic cells. Among normal BM cells, CD117 expression has been found in about half of the CD34 precursors including progenitors committed to the erythroid, granulo-monocytic, and megakaryocytic cell lineages. CD117 Protein, Human, Recombinant (His & Avi), Biotinylated is expressed in HEK293 mammalian cells with C-His-Avi tag. The predicted molecular weight is 58.60 kDa and the accession number is P10721-2.
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 5 μg | ¥ 568 | 3日内发货 | |
| 10 μg | ¥ 928 | 3日内发货 | |
| 20 μg | ¥ 1,530 | 3日内发货 | |
| 50 μg | ¥ 2,970 | 3日内发货 | |
| 100 μg | ¥ 5,180 | 现货 | |
| 200 μg | ¥ 9,390 | 5日内发货 | |
| 500 μg | ¥ 20,700 | 5日内发货 |
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纯度: > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
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产品介绍
生物活性
化学信息
储存&溶解度
| 产品描述 | The c-kit proto-oncogen (CD 117) has been shown to be present in several cell types including normal and neoplastic hemopoietic cells. Among normal BM cells, CD117 expression has been found in about half of the CD34 precursors including progenitors committed to the erythroid, granulo-monocytic, and megakaryocytic cell lineages. CD117 Protein, Human, Recombinant (His & Avi), Biotinylated is expressed in HEK293 mammalian cells with C-His-Avi tag. The predicted molecular weight is 58.60 kDa and the accession number is P10721-2. |
| 生物活性 | Immobilized Biotinylated Human CD117, His-Avi Tag at 1 μg/ml (100 μl/well) on the streptavidin precoated plate (5 μg/ml). Dose response curve for Anti-CD117 Antibody, hFc Tag with the EC50 of 4.6 ng/ml determined by ELISA (QC Test). Immobilized Human SCF, No Tag at 5 μg/ml (100 μl/well) on the plate. Dose response curve for Biotinylated Human CD117, His Tag with the EC50 of 6.8 μg/ml determined by ELISA. |
| 研究背景 | The c-kit proto-oncogen (CD 117) has been shown to be present in several cell types including normal and neoplastic hemopoietic cells. Among normal BM cells, CD117 expression has been found in about half of the CD34 precursors including progenitors committed to the erythroid, granulo-monocytic, and megakaryocytic cell lineages. |
| 种属 | Human |
| 表达系统 | HEK293 Cells |
| 标签 | C-His-Avi |
| 蛋白编号 | P10721-2 |
| 蛋白构建 | Gln26-Pro520 |
| 蛋白纯度 | > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
| 蛋白性状 | Lyophilized powder |
| 缓冲液 | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS (pH 7.4). Typically, 8% trehalose is incorporated as a protective agent before lyophilization. |
| 复溶方法 | Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing. |
| 别名 | SCFR, PBT, KIT, EC 2.7.10.1, EC 2.7.10, c-kit, ckit, CD117 |
| 内毒素 | < 1 EU/μg by the LAL method. |
| 分子量 | 58.60 kDa (predicted). Due to glycosylation, the protein migrates to 75-105 kDa based on Tris-Bis PAGE result. |
| 颜色 | White |
| 物理性状 | Lyophilized powder |
| 运输方式 | In general, Lyophilized powders are shipping with blue ice. |
| 存储 | It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
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