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Cathepsin C Protein, Mouse, Recombinant (His)

产品编号 TMPY-07185

Cathepsin C Protein, Mouse, Recombinant (His) is expressed in HEK293 Cells. The accession number is P97821.

Cathepsin C Protein, Mouse, Recombinant (His)

Cathepsin C Protein, Mouse, Recombinant (His)

产品编号 TMPY-07185
Cathepsin C Protein, Mouse, Recombinant (His) is expressed in HEK293 Cells. The accession number is P97821.
规格价格库存数量
50 μg
¥ 3,820
期货
500 μg
¥ 24,900
期货
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实验操作小课堂
常见问题解答
抑制剂能否用于细胞实验?
可以的,我们的抑制剂都可以用于细胞/体外实验。但有些化合物还没有用于细胞实验的文献,这种情况下,建议您先做预实验,确保研究的可行性。
动物不能耐受 DMSO,给药时 DMSO量应该如何控制?
对于普通的老鼠,DMSO 的浓度应控制在 10% 以下,对于裸鼠、转基因小鼠、耐受性弱的老鼠等,DMSO 浓度需控制在2%以下。对于首次操作的抑制剂,建议先做溶剂阴性对照,确认溶解对动物无非特异性影响。
细胞实验中,抑制剂如何溶解?
用 DMSO 配置较高浓度母液,母液存于 -80℃,建议多分装几管,一周内使用的存于 4℃,避免反复冻融。实验时用 pbs、生理盐水、培养基、水这些稀释,细胞实验DMSO的终浓度不超过 0.1%。如果超过 0.1%,则需要在做预试验的时候,先进行溶剂阴性对照实验,排除溶剂对细胞的影响。
抑制剂是否可以高温灭菌?
大部分产品均通过化学方法合成,反应条件一般在 50-80℃ 之间,因此不建议使用高温灭菌法进行灭菌,建议用 0.22 μm 滤膜过滤灭菌。
超声一般需要多久?
超声时长和溶解的量和浓度都有关,一般建议半个小时左右,如果浓度接近最大溶解度,或者需溶解的粉末较多,建议延长超声时间,并辅以加热。
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产品信息

生物活性
Activity testing is in progress. It is theoretically active, but we cannot guarantee it. If you require protein activity, we recommend choosing the eukaryotic expression version first.
产品描述
Cathepsin C Protein, Mouse, Recombinant (His) is expressed in HEK293 Cells. The accession number is P97821.
种属
Mouse
表达系统
HEK293 Cells
标签C-His
蛋白编号P97821
别名
DPPI,DPP1,Ctsc,CatC
蛋白构建
A DNA sequence encoding the mouse CTSC (NP_034112.3) precursor (Met1-Leu462) was expressed with a polyhistidine tag at the C-terminus.
蛋白纯度
≥ 95% as determined by SDS-PAGE. ≥ 90% as determined by SEC-HPLC.
分子量51.2 kDa (predicted); 51.2 kDa (reducing contition)
内毒素< 1.0 EU per μg of the protein as determined by the LAL method
缓冲液Supplied as sterile 20mM MES, 150mM Nacl, 10% Glycerol, 0.02% Tween 20, 10% Trehalose, pH 5.5. Please contact us for any concerns or special requirements. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
存储
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
运输方式In general, Lyophilized powders are shipping with blue ice. Solutions are shipping with dry ice.
研究背景
Cathepsins are proteases found in many types of cells conserved in all animals, which have a vital role in mammalian cellular turnover such as bone resorption. The lysosomal cysteine protease Cathepsin C (CTSC), also known as dipeptidyl peptidase I (DPPI/DPP1), activates a number of granule-associated serine proteases with pro-inflammatory and immune functions by removal of their inhibitory N-terminal dipeptides. This lysosomal exo-cysteine protease belonging to the peptidase C1 family. Active cathepsin C is found in lysosomes as a 200-kDa multimeric enzyme. Subunits constituting this assembly all arise from the proteolytic cleavage of a single precursor giving rise to three peptides: the propeptide, the alpha- and the beta-chains. It is a central coordinator for activation of many serine proteases in immune/inflammatory cells. Defects in the Cathepsin C have been shown to be a cause of Papillon-Lefevre disease, an autosomal recessive disorder characterized by palmoplantar keratosis and periodontitis. Cathepsin C plays a key role in the activation of several degradative enzymes linked to tissue destruction in inflammatory diseases. Thus, it is a therapeutic target for the treatment of a number of inflammatory and autoimmune diseases.

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