生物活性

产品描述	Carbonic Anhydrase 4 Protein, Human, Recombinant (hFc) is expressed in HEK293 Cells. The accession number is P22748-1.
生物活性	Activity has not been tested. It is theoretically active, but we cannot guarantee it.
研究背景	Carbonic anhydrase IV is one of 12 active human isozymes and one of four expressed on the extracellular surfaces of certain endothelial and epithelial cells. It is unique in being attached to the plasma membrane by a glycosyl-phosphatiydyl-inositol (GPI) anchor rather than by a membrane-spanning domain. It is also uniquely resistant to high concentrations of sodium dodecyl sulfate (SDS), which allows purification from tissues by inhibitor affinity chromatography without contamination by other isozymes.
种属	Human
表达系统	HEK293 Cells
标签	C-hFc (IgG1)
蛋白编号	P22748-1
蛋白构建	Ala19-Ser284
蛋白纯度	> 95% as determined by Bis-Tris PAGE; > 95% as determined by HPLC
缓冲液	Lyophilized from 0.22 μm filtered solution in 25 mM Tris, 150 mM NaCl (pH 7.5). Normally 8% trehalose is added as protectant before lyophilization.
复溶方法	Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
内毒素	< 0.1 EU/μg of the protein as determined by the LAL method.

化学信息

分子量	56.26 kDa (Predicted); 57-67 kDa (Reducing condition, due to glycosylation)

储存&溶解度

运输方式

In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.

存储

It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

实际储存温度请以COA为准

Carbonic Anhydrase 4 Protein, Human, Recombinant (hFc)

Carbonic Anhydrase 4 Protein, Human, Recombinant (hFc)

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