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CAMKI Protein, Human, Recombinant

CAMKI Protein, Human, Recombinant

产品编号 TMPY-04415
别名: calcium/calmodulin-dependent protein kinase I, CAMKI

CAMKI Protein, Human, Recombinant is expressed in E. coli expression system. The predicted molecular weight is 41.5 kDa and the accession number is B0YIY3.

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CAMKI Protein, Human, Recombinant
规格 价格/CNY 货期 数量
50 μg ¥ 2,530 5日内发货
药物设计专题培训
千万补贴 助力科研
BCA蛋白浓度测定试剂盒限时半价
产品目录号及名称: CAMKI Protein, Human, Recombinant (TMPY-04415)
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生物活性
技术参数
产品性质
参考文献
生物活性 The specific activity was determined to be >70 nmol/min/mg using Autocamtide-2 synthetic peptide (KKALRRQETVDAL-amide) as substrate.
产品描述 CAMKI Protein, Human, Recombinant is expressed in E. coli expression system. The predicted molecular weight is 41.5 kDa and the accession number is B0YIY3.
种属 Human
表达系统 E. coli
标签 Tag Free
蛋白编号 B0YIY3
别名 calcium/calmodulin-dependent protein kinase I, CAMKI
蛋白构建 The Human CAMK1 (NP_003647.1) (Leu 2-Leu 370) was expressed and purified, with two additional amino acids (Gly & Pro) at the N-terminus.
蛋白纯度 > 85 % as determined by SDS-PAGE
分子量 41.5 kDa (predicted)
内毒素 Please contact us for more information.
缓冲液 Supplied as sterile 50 mM Tirs, 150 mM NaCl, 10% glycerol, pH 7.5.
复溶方法 A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
存储

It is recommended to store the product under sterile conditions at -20℃ to -80℃. Samples are stable for up to 12 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

运输方式

Kinases are highly recommended to be shipped at frozen temperature with blue ice or dry ice.

研究背景 Calcium/calmodulin-dependent protein kinase orCaM kinases are serine/threonine-specific protein kinasesthat are primarily regulated by the Calcium/calmodulin complex. These kinases show a memory effect on activation. CaM kinases activity can outlast the intracellular calcium transient that is needed to activate it. Inneurons, this property is important for the induction of synaptic plasticity. Pharmacological inhibition of CaM kinases II blocks the induction oflong-term potentiation. Upon activation, CaM kinases II phosphorylates postsynaptic glutamate receptors and changes the electrical properties of the synapse. Calcium/calmodulin-dependent protein kinase type 1D, also known as CaM kinase I delta, CaM kinase ID, CaMKI-like protein kinase, CKLiK and CAMK1D, is a member of theprotein kinase superfamily and CaMK subfamily. It contains oneprotein kinase domain. CAMK1D is broadly expressed. It is highly and mostly expressed in polymorphonuclear leukocytes (neutrophilic and eosinophilic granulocytes) while little or no expression is observed in monocytes and lymphocytes. Engineered overexpression of CAMK1D in non-tumorigenic breast epithelial cells led to increased cell proliferation, and molecular and phenotypic alterations indicative of epithelial-mesenchymal transition (EMT), including loss of cell-cell adhesions and increased cell migration and invasion. CAMK1D is a potential therapeutic target with particular relevance to clinically unfavorable basal-like tumors.

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Keywords

CAMKI Protein, Human, Recombinant calcium/calmodulin-dependent protein kinase I CAMKI recombinant recombinant-proteins proteins protein

 

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