CALCA/CGRP Protein, Mouse, Recombinant (hFc)

Specific fragments of methylation changes in the target gene (Calca) were identified by IGV analysis. CGRP was applied to compare the effects on ASCs-T2DM morphology via phalloidin staining, proliferation through CCK-8 assay, and osteogenic differentiation with osteogenic staining, qPCR, and repair of calvarial defect. Furthermore, 5-azacytidine (5-az) was used to intervene ASCs-T2DM to verify the relationship between the methylation level of the target fragment and expression of Calca.

规格	价格/CNY		数量
100 μg	￥ 2,660	现货
500 μg	￥ 10,600	5日内发货
1 mg	￥ 17,800	5日内发货

规格

价格/CNY

货期

数量

100 μg

￥ 2,660

现货

500 μg

￥ 10,600

5日内发货

1 mg

￥ 17,800

5日内发货

产品描述

种属	Mouse
表达系统	HEK293 Cells
标签	C-hFc
蛋白编号	Q99JA0
别名	CGRP, Katacalcin, PDN-21, CGRP-I, CGRP1, Calcitonin 1, Calcitonin, CCP, CALCA, PCT, KC, CT, Procalcitonin, CALC1
蛋白构建	Val26-Phe119
蛋白纯度	> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
分子量	36.9 kDa (predicted). Due to glycosylation, the protein migrates to 42-46 kDa based on Tris-Bis PAGE result.

内毒素	< 1 EU/μg by the LAL method.
缓冲液	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS (pH 7.4). Typically, 8% trehalose is incorporated as a protective agent before lyophilization.
复溶方法	Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
存储	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
运输方式	In general, Lyophilized powders are shipping with blue ice.
研究背景	Specific fragments of methylation changes in the target gene (Calca) were identified by IGV analysis. CGRP was applied to compare the effects on ASCs-T2DM morphology via phalloidin staining, proliferation through CCK-8 assay, and osteogenic differentiation with osteogenic staining, qPCR, and repair of calvarial defect. Furthermore, 5-azacytidine (5-az) was used to intervene ASCs-T2DM to verify the relationship between the methylation level of the target fragment and expression of Calca.