BAFF/TNFSF13B Protein, Human, Recombinant (His)

BAFF/TNFSF13B Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with N-6xHis tag. The predicted molecular weight is 22 KDa and the accession number is Q9Y275.

规格	价格/CNY		数量
10 μg	￥ 850	5日内发货
50 μg	￥ 2,570	5日内发货
500 μg	￥ 14,200	5日内发货
1 mg	￥ 21,300	5日内发货

规格

价格/CNY

货期

数量

10 μg

￥ 850

5日内发货

50 μg

￥ 2,570

5日内发货

500 μg

￥ 14,200

5日内发货

1 mg

￥ 21,300

5日内发货

生物活性	1. Loaded Human BCMA-Fc on Protein A Biosensor, can bind Human BAFF-His with an affinity constant of 0.02 nM as determined in BLI assay. (Regularly tested)2 . Immobilized Human BAFF-His at 10μg/ml (100 μl/well) can bind Mouse BCMA-Fc . The ED50 of Recombinant Mouse BCMA-Fc is 0.016 ug/ml. (Regularly tested)
产品描述	BAFF/TNFSF13B Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with N-6xHis tag. The predicted molecular weight is 22 KDa and the accession number is Q9Y275.

种属	Human
表达系统	Human Cells
标签	N-6His
蛋白编号	Q9Y275
别名	B lymphocyte stimulator, TNF- and APOL-related leukocyte expressed ligand 1, TALL-1, BLyS, BAFF, Tumor necrosis factor ligand superfamily member 13B, Dendritic cell-derived TNF-like molecule, B-cell-activating factor
氨基酸序列	Ala134-Leu285
蛋白构建	Recombinant Human TNF Ligand Superfamily Member 13B is produced by our Mammalian expression system and the target gene encoding Ala134-Leu285 is expressed with a 6His tag at the N-terminus.
蛋白纯度	Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
分子量	22 KDa, reducing conditions

内毒素	Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test.
缓冲液	Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
复溶方法	Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
存储	Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
运输方式	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature listed below.
研究背景	TNFSF13B/TNFSF20 belongs to the tumor necrosis factor family. It abundantly is expressed in peripheral blood Leukocytes and is specifically expressed in monocytes and macrophages. Also found in the spleen, lymph node, bone marrow, T-cells and dendritic cells. A lower expression seen in placenta, heart, lung, fetal liver, thymus, and pancreas. Isoform 2 is expressed in many myeloid cell lines. Cytokine that binds to TNFRSF13B/TACI and TNFRSF17/BCMA. TNFSF13/APRIL binds to the same 2 receptors. Together, they form a 2 ligands -2 receptors pathway involved in the stimulation of B- and T-cell function and the regulation of humoral immunity. A third B-cell specific BAFF-receptor (BAFFR/BR3) promotes the survival of mature B-cells and the B-cell response. Isoform 2 seems to inhibit isoform 1 secretion and bioactivity. Isoform 3 acts as a transcription factor for its own parent gene, in association with NF-kappa-B p50 subunit, at least in autoimmune and proliferative B-cell diseases. The presence of Delta4BAFF is essential for soluble BAFF release by IFNG/IFN-gamma-stimulated monocytes and for B-cell survival. It can directly or indirectly regulate the differential expression of a large number of genes involved in the innate immune response and the regulation of apoptosis. Isoform 2 heteromultimerizes with isoform 1, probably limiting the amount of functional isoform 1 on the cell surface. Isoform 3 is unlikely form trimers or bind to BAFF receptors. Mature human BAFF consists of a 46 amino acid (aa) cytoplasmic domain, a 21 aa transmembrane segment, and a 218 aa extracellular domain (ECD) with a stalk region and one TNF-like domain. Within aa 134-285 of the ECD, human BAFF shares 72% aa sequence identity with mouse BAFF.

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BAFF/TNFSF13B Protein, Human, Recombinant (His)

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