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B7-H3 (4Ig)/B7-H3b Protein, Human, Recombinant (His) is expressed in HEK293 Cells. The accession number is Q5ZPR3-1.
别名 CD276 molecule, B7RP-2, B7-H3, B7H3, 4Ig-B7-H3
B7-H3 (4Ig)/B7-H3b Protein, Human, Recombinant (His) is expressed in HEK293 Cells. The accession number is Q5ZPR3-1.

| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 5 μg | ¥ 316 | 6-8日内发货 | |
| 10 μg | ¥ 492 | 6-8日内发货 | |
| 20 μg | ¥ 818 | 5日内发货 | |
| 50 μg | ¥ 1,580 | 5日内发货 | |
| 100 μg | ¥ 2,690 | 5日内发货 | |
| 200 μg | ¥ 4,760 | 5日内发货 | |
| 500 μg | ¥ 10,000 | 5日内发货 |
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| 产品描述 | B7-H3 (4Ig)/B7-H3b Protein, Human, Recombinant (His) is expressed in HEK293 Cells. The accession number is Q5ZPR3-1. |
| 生物活性 | Immobilized Human B7-H3 (4Ig), His Tag at 0.5 μg/ml (100 μl/well) on the plate. Dose response curve for Anti-B7-H3 Antibody, hFc Tag with the EC50 of 5.0 ng/ml determined by ELISA. (QC Test) Loaded Anti-B7-H3 Ab, hFc Tag on ProA-Biosensor can bind Human B7-H3 (4Ig), His Tag with an affinity constant of 4.59 nM as determined in BLI assay . |
| 研究背景 | B7-H3, a member of the B7 family of immunomodulatory molecules, is overexpressed in a wide range of solid cancers.B7-H3 binds to activated T cells via an as yet unidentified receptor. In assays using sub-optimal amount so anti-CD3 stimulation, 2Ig‑B7‑H3 enhances T cell proliferation, T cell interferon-gamma (IFN-gamma) production, and cytotoxic T cells induction. |
| 种属 | Human |
| 表达系统 | HEK293 Cells |
| 标签 | C-His |
| 蛋白编号 | Q5ZPR3-1 |
| 蛋白构建 | Gly27-Thr461 |
| 蛋白纯度 | > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
| 蛋白性状 | Lyophilized powder |
| 缓冲液 | Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| 复溶方法 | Reconstitute the lyophilized protein in sterile deionized water. The product concentration should not be less than 100 μg/mL. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing. |
| 别名 | CD276 molecule, B7RP-2, B7-H3, B7H3, 4Ig-B7-H3 |
| 内毒素 | < 0.1 EU/μg of the protein as determined by the LAL method. |
| 分子量 | 48.25 kDa (Predicted); 68-78 kDa (Due to glycosylation) |
| 运输方式 | In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice. |
| 存储 | Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. 实际储存温度请以COA为准 |
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