生物活性

产品描述	B7-H2/ICOSLG Protein, Rat, Recombinant (His) is expressed in HEK293 Cells. The accession number is F1LVL2.
生物活性	Immobilized Rat B7-H2, His Tag at 1 μg/ml (100 μl/well) on the plate. Dose response curve for Mouse ICOS, hFc Tag with the EC50 of 28.2 ng/ml determined by ELISA (QC Test).
研究背景	B7-H2, also known as B7-related protein (B7RP1), ICOS Ligand, and CD275, is an approximately 60 kDa transmembrane glycoprotein in the B7 family of immune regulatory molecules. B7-H2 is a ligand for the T-cell-specific cell surface receptor ICOS. Acts as a costimulatory signal for T-cell proliferation and cytokine secretion; induces also B-cell proliferation and differentiation into plasma cells.
种属	Rat
表达系统	HEK293 Cells
标签	C-His
蛋白编号	F1LVL2
蛋白构建	Glu25-Lys261
蛋白纯度	> 95% as determined by Bis-Tris PAGE; > 90% as determined by HPLC
缓冲液	Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
复溶方法	Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
内毒素	< 0.1 EU/μg of the protein as determined by the LAL method.

化学信息

分子量	28.52 kDa (Predicted); 50-70 kDa (Reducing condition, due to glycosylation)

储存&溶解度

运输方式

In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.

存储

It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

实际储存温度请以COA为准

B7-H2/ICOSLG Protein, Rat, Recombinant (His)

B7-H2/ICOSLG Protein, Rat, Recombinant (His)

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