生物活性

产品描述	APRIL/TNFSF13 Trimer Protein, Cynomolgus, Recombinant (His & Avi, Flag), Biotinylated is expressed in HEK293 Cells. The accession number is A0A2K5TJA1.
生物活性	Immobilized Biotinylated Cynomolgus APRIL (Trimer), His-Avi Tag at 1 μg/ml (100 μl/Well) on streptavidin (5 μg/ml) precoated plate. Dose response curve for Cynomolgus BCMA, hFc Tag with the EC50 of 2.0 ng/ml determined by ELISA.
研究背景	The APRIL (a proliferation-inducing ligand), also known as TNFSF13, TALL2, TRDL1, and CD256, is a member of the TNF ligand superfamily.Both APRIL and its close relative BAFF bind and signal through the TNF superfamily receptors TACI and BCMA, while BAFF additionally functions through BAFFR.
种属	Cynomolgus
表达系统	HEK293 Cells
标签	N-His-Avi-Flag
蛋白编号	A0A2K5TJA1
蛋白构建	Lys112-Leu250 trimer design
蛋白纯度	> 95% as determined by Bis-Tris PAGE; > 90% as determined by HPLC
缓冲液	Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, 200 mM L-arginine (pH 8.0). Normally 8% mannitol is added as protectant before lyophilization.
复溶方法	Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
内毒素	< 1 EU/μg of the protein as determined by the LAL method.

化学信息

分子量	52.1 kDa (Predicted); 56-65 kDa (Reducing condition, due to glycosylation)

储存&溶解度

运输方式

In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.

存储

It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

实际储存温度请以COA为准

APRIL/TNFSF13 Trimer Protein, Cynomolgus, Recombinant (His & Avi, Flag), Biotinylated

APRIL/TNFSF13 Trimer Protein, Cynomolgus, Recombinant (His & Avi, Flag), Biotinylated

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