Ameloblastin Protein, Human, Recombinant (His)

产品编号 TMPJ-00579

Ameloblastin (AMBN) is a member of the Ameloblastin family. AMBN is a secreted protein and is specially expressed in ameloblast, localizing to the Tomes processes of secretory ameloblasts and in the sheath space between rod-interrod enamel Mutations of this protein may be associated with dentinogenesis imperfect and autosomal dominant amylogenesis imperfect. Ameloblastin may play an important role in the formation and mineralization of the enamel matrix. Biochemically, it is classified as an intrinsically disordered protein (IDP). Its biological role remains largely unknown.

Ameloblastin Protein, Human, Recombinant (His)

产品编号 TMPJ-00579

规格	价格	库存
10 μg	¥ 1,170	5日内发货
50 μg	¥ 3,470	5日内发货
500 μg	¥ 12,100	5日内发货
1 mg	¥ 17,400	5日内发货

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常见问题解答

问为什么某些重组蛋白溶液中还需要添加载体蛋白？

答载体蛋白通常被用于提高蛋白的稳定性，防止蛋白在冷冻或解冻过程中粘附在管壁上。塑料管对蛋白有一定的吸附能力，这可能导致蛋白和管壁之间难以分离，导致溶液中蛋白的实际浓度下降，从而影响其活性。为减少这种损失，在长期保存重组蛋白产品之前，建议添加常用的载体蛋白溶液，例如0.1% BSA（牛血清白蛋白）、5% HSA（人血清白蛋白）、10% FBS（胎牛血清）或 5% 海藻糖等。如果重组蛋白的浓度较低，强烈建议添加适量的载体蛋白。

问重组蛋白产品是如何运输的？

答大部分重组蛋白产品为冻⼲粉，对温度不敏感，在室温下稳定，一般室温或蓝冰运输。对于液体类蛋白，一般采用⼲冰运输。如果您收到产品时发现冰盒已经融化，无需担心，蛋白可以正常使用，短时间内不会影响产品质量。如果特定蛋白在室温下不稳定，我们会提供相应的低温运输方式，以确保产品质量不受影响。

问重组蛋白产品该如何保存？

答1) 存储条件:重组蛋白产品收到后，如不立即使用，应当保存在 -20℃ 至 -80℃ 的条件下。冻干粉可稳定保存 12 个月以上，液体类产品在 -80℃ 条件下可保存 6-12 个月。已复溶的重组蛋白样品需尽快用完，如果不能在一周内用完，需在 -20℃ 至 -80℃ 保存，可保存 3 个月以上\n2) 避免反复融冻:重组蛋白产品应注意避免反复冻融，建议将重组蛋白产品分装成多份。分装冻存时，建议选择含有一定浓度载体蛋白的溶液或培养基复溶产品。产品浓度不低于 100 ug/mL，且每管的体积不小于 20 uL。

问重组蛋白有吗？

答您需要什么种属的蛋白呢，对表达系统和标签有要求吗，用于什么实验？

问如何将冻干的重组蛋白配置为储备液？

答在打开盖⼦之前，先使用 10000-12000 rpm 的转速进行离心，20-30 秒，将附着在管盖或管壁上的冻⼲粉收集到管底，以避免损失。大多数蛋白可通过加入无菌蒸馏水来重新溶解。如果需要除水以外的其他稀释剂，产品 COA 中会对其进行说明。一般储备液浓度建议不要低于 100 μg/mL，分装储存避免反复冻融。

产品信息

生物活性	Activity has not been tested. It is theoretically active, but we cannot guarantee it. If you require protein activity, we recommend choosing the eukaryotic expression version first.
产品描述	Ameloblastin (AMBN) is a member of the Ameloblastin family. AMBN is a secreted protein and is specially expressed in ameloblast, localizing to the Tomes processes of secretory ameloblasts and in the sheath space between rod-interrod enamel Mutations of this protein may be associated with dentinogenesis imperfect and autosomal dominant amylogenesis imperfect. Ameloblastin may play an important role in the formation and mineralization of the enamel matrix. Biochemically, it is classified as an intrinsically disordered protein (IDP). Its biological role remains largely unknown.
种属	Human
表达系统	HEK293 Cells
标签	C-6xHis
蛋白编号	AAI06932.1
别名	Ameloblastin,AMBN
氨基酸序列	Val27-Pro447
蛋白构建	Val27-Pro447
分子量	60-80 KDa (reducing condition)
内毒素	< 0.1 ng/µg (1 EU/µg) as determined by LAL test.
缓冲液	Lyophilized from a solution filtered through a 0.22 μm filter, containing 20 mM PB, 150 mM NaCl, pH 7.4.
复溶方法	Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
存储	Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
运输方式	In general, Lyophilized powders are shipping with blue ice. Solutions are shipping with dry ice.
研究背景	Ameloblastin (AMBN) is a member of the Ameloblastin family. AMBN is a secreted protein and is specially expressed in ameloblast, localizing to the Tomes processes of secretory ameloblasts and in the sheath space between rod-interrod enamel Mutations of this protein may be associated with dentinogenesis imperfect and autosomal dominant amylogenesis imperfect. Ameloblastin may play an important role in the formation and mineralization of the enamel matrix. Biochemically, it is classified as an intrinsically disordered protein (IDP). Its biological role remains largely unknown.

Ameloblastin Protein, Human, Recombinant (His)

Ameloblastin Protein, Human, Recombinant (His)

产品信息

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