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Allergin-1 Protein, Mouse, Recombinant (His & Myc)

产品编号 TMPH-02499

Immunoglobulin-like receptor which plays an inhibitory role in degranulation of mast cells. Negatively regulates IgE-mediated mast cell activation and suppresses the type I immediate hypersensitivity reaction. Allergin-1 Protein, Mouse, Recombinant (His & Myc) is expressed in E. coli expression system with N-10xHis and C-Myc tag. The predicted molecular weight is 20.1 kDa and the accession number is Q3TB92.

Allergin-1 Protein, Mouse, Recombinant (His & Myc)

Allergin-1 Protein, Mouse, Recombinant (His & Myc)

产品编号 TMPH-02499
Immunoglobulin-like receptor which plays an inhibitory role in degranulation of mast cells. Negatively regulates IgE-mediated mast cell activation and suppresses the type I immediate hypersensitivity reaction. Allergin-1 Protein, Mouse, Recombinant (His & Myc) is expressed in E. coli expression system with N-10xHis and C-Myc tag. The predicted molecular weight is 20.1 kDa and the accession number is Q3TB92.
规格价格库存数量
20 μg
¥ 2,290
20日内发货
100 μg
¥ 4,750
20日内发货
1 mg
¥ 16,000
20日内发货
大包装 & 定制
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实验操作小课堂
常见问题解答
如何选择合适的溶剂?
常用的溶剂包括DMSO、水、乙醇等。建议您根据抑制剂给出的溶解度参考,选择合适的溶剂。如果该抑制剂可以溶于DMSO,建议使用没有吸潮新开封的 DMSO,如果有潮气污染,很容易带来抑制剂降解或难溶的问题。如果该抑制剂可以溶于水,建议您根据实验类型选择无菌水、生理盐水、无菌 PBS 、培养基等作为溶剂。
超声一般需要多久?
超声时长和溶解的量和浓度都有关,一般建议半个小时左右,如果浓度接近最大溶解度,或者需溶解的粉末较多,建议延长超声时间,并辅以加热。
产品的溶解度是多少?
建议您先查询TargetMol官网、陶术官网,看该抑制剂的产品介绍,找到溶解度信息。如果没有相应信息,建议联系技术对该化合物的溶解度进行测定。
加药后检测结果没有作用,是不是产品质量有问题?
产品质量没有问题,都是经过两次严格质检的,HNMR确定产品结构,HPLC确定化合物纯度。同一个抑制剂由于实验材料(细胞、动物)的不同,IC50 和实验效果也是不一样的,不能照搬文献报道的方法,应该根据自己的实验设立浓度梯度,得到一个最适合的浓度。
PEG300 可以用 PEG400 替代吗?
PEG300 具有适度的粘稠度,有利于药物的溶解和给药过程,同时也能够提供良好的耐受性,不会对动物造成不良影响。PEG400 可以替代 PEG300。 PEG600 不是很建议,因为熔点接近常温。
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产品信息

生物活性
Activity has not been tested. It is theoretically active, but we cannot guarantee it. If you require protein activity, we recommend choosing the eukaryotic expression version first.
产品描述
Immunoglobulin-like receptor which plays an inhibitory role in degranulation of mast cells. Negatively regulates IgE-mediated mast cell activation and suppresses the type I immediate hypersensitivity reaction. Allergin-1 Protein, Mouse, Recombinant (His & Myc) is expressed in E. coli expression system with N-10xHis and C-Myc tag. The predicted molecular weight is 20.1 kDa and the accession number is Q3TB92.
种属
Mouse
表达系统
E. coli
标签N-10xHis, C-Myc
蛋白编号Q3TB92
别名
Milr1,Mca32,Mast cell immunoglobulin-like receptor 1,Mast cell antigen 32 (MCA-32;Mast cell Ag-32),Gm885,Allergy inhibitory receptor 1,Allergin-1
氨基酸序列
ITLQNAAVDCTRVENNELPSPNLNSSMNVVRMGQNVSLSCSTKNTSVDITYSLFWGTKYLESKRRRGGAVDFHLRISNANESGPYKCKVNVSNLMKYSQDFNFTMAKDESCPSCRLS
蛋白构建
34-150 aa
蛋白纯度
> 85% as determined by SDS-PAGE.
分子量20.1 kDa (predicted)
内毒素< 1.0 EU/μg of the protein as determined by the LAL method.
缓冲液Tris-based buffer, 50% glycerol
复溶方法
A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
存储
Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
运输方式In general, Lyophilized powders are shipping with blue ice. Solutions are shipping with dry ice.
研究背景
Immunoglobulin-like receptor which plays an inhibitory role in degranulation of mast cells. Negatively regulates IgE-mediated mast cell activation and suppresses the type I immediate hypersensitivity reaction.

SCI 文献

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