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AGRP Protein, Mouse, Recombinant (His)

产品编号 TMPY-02269

AGRP Protein, Mouse, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 13.7 kDa and the accession number is P56473.

AGRP Protein, Mouse, Recombinant (His)

AGRP Protein, Mouse, Recombinant (His)

产品编号 TMPY-02269
AGRP Protein, Mouse, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 13.7 kDa and the accession number is P56473.
规格价格库存数量
200 μg
¥ 5,750
5日内发货
大包装 & 定制
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产品信息

生物活性
Activity testing is in progress. It is theoretically active, but we cannot guarantee it. If you require protein activity, we recommend choosing the eukaryotic expression version first.
产品描述
AGRP Protein, Mouse, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 13.7 kDa and the accession number is P56473.
种属
Mouse
表达系统
HEK293 Cells
标签C-His
蛋白编号P56473
别名
Art,Agrt,agouti related neuropeptide
蛋白构建
A DNA sequence encoding the extracellular domain of mouse AGRP (P56473) (Met 1-Thr 131) was expressed, with a C-terminal polyhistidine tag. Predicted N terminal: Val 21
蛋白纯度
> 80 % as determined by SDS-PAGE
分子量13.7 kDa (predicted); 20 kDa (reducing conditions)
内毒素< 1.0 EU/μg of the protein as determined by the LAL method.
缓冲液Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
复溶方法
A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
存储
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
运输方式In general, Lyophilized powders are shipping with blue ice.
研究背景
Agouti Related Protein (AGRP, or AGRT), is an endogenous antagonist of the melanocortin receptors MC3R and MC4R found in the hypothalamus and exhibits potent orexigenic activity. AGRP can act as a competitive antagonist to proopiomelanocortin (POMC)-derived peptides at the melanocortin-4 receptor (MC4R), and that this homeostatic mechanism is important as a means of coordinating appetite with perceived metabolic requirement. AGRP is upregulated by fasting while intracerebroventricular injections of synthetic AGRP lead to increased appetite and food intake. Thus, AGRP is a powerful orexigenic peptide that increases food intake when ubiquitously overexpressed or when administered centrally.

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