Adeno-associated virus 2 (isolate Srivastava/1982) Rep78 Protein (His & MBP)

产品编号 TMPH-00028

Plays an essential role in the initiation of viral DNA synthesis. Binds specifically to an inverted terminal repeat element (ITR) on the 3' and 5' ends of the viral DNA, where it cleaves a site specifically to generate a priming site for initiation of the synthesis of a complementary strand. Plays also a role as transcriptional regulator, DNA helicase and as key factors in site-specific integration of the viral genome. Regulates host PKA activity by interacting with host PRKX as a mechanism of interfering with helper virus propagation and promoting its own replication. Inhibits the host cell cycle G1/S, S and G2/M transitions. These arrests may provide essential cellular factors for viral DNA replication.

Adeno-associated virus 2 (isolate Srivastava/1982) Rep78 Protein (His & MBP)

产品编号 TMPH-00028

规格	价格	库存
20 μg	¥ 3,670	20日内发货
100 μg	待询	20日内发货
1 mg	待询	20日内发货

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常见问题解答

问重组蛋白产品是如何运输的？

答大部分重组蛋白产品为冻⼲粉，对温度不敏感，在室温下稳定，一般室温或蓝冰运输。对于液体类蛋白，一般采用⼲冰运输。如果您收到产品时发现冰盒已经融化，无需担心，蛋白可以正常使用，短时间内不会影响产品质量。如果特定蛋白在室温下不稳定，我们会提供相应的低温运输方式，以确保产品质量不受影响。

问重组蛋白有吗？

答您需要什么种属的蛋白呢，对表达系统和标签有要求吗，用于什么实验？

问蛋白能保证活性吗？

答我们有现货的产品，有活性数据的话会展示在官网生物活性那栏，没有数据的也不代表就一定没有活性，是我们还没有建立活性检测方法，没有进行测试，但是我们的蛋白都是优先按照有活性的方法去表达纯化的。

问如何将冻干的重组蛋白配置为储备液？

答在打开盖⼦之前，先使用 10000-12000 rpm 的转速进行离心，20-30 秒，将附着在管盖或管壁上的冻⼲粉收集到管底，以避免损失。大多数蛋白可通过加入无菌蒸馏水来重新溶解。如果需要除水以外的其他稀释剂，产品 COA 中会对其进行说明。一般储备液浓度建议不要低于 100 μg/mL，分装储存避免反复冻融。

问重组蛋白产品该如何保存？

答1) 存储条件:重组蛋白产品收到后，如不立即使用，应当保存在 -20℃ 至 -80℃ 的条件下。冻干粉可稳定保存 12 个月以上，液体类产品在 -80℃ 条件下可保存 6-12 个月。已复溶的重组蛋白样品需尽快用完，如果不能在一周内用完，需在 -20℃ 至 -80℃ 保存，可保存 3 个月以上\n2) 避免反复融冻:重组蛋白产品应注意避免反复冻融，建议将重组蛋白产品分装成多份。分装冻存时，建议选择含有一定浓度载体蛋白的溶液或培养基复溶产品。产品浓度不低于 100 ug/mL，且每管的体积不小于 20 uL。

产品信息

生物活性	Activity has not been tested. It is theoretically active, but we cannot guarantee it. If you require protein activity, we recommend choosing the eukaryotic expression version first.
产品描述	Plays an essential role in the initiation of viral DNA synthesis. Binds specifically to an inverted terminal repeat element (ITR) on the 3' and 5' ends of the viral DNA, where it cleaves a site specifically to generate a priming site for initiation of the synthesis of a complementary strand. Plays also a role as transcriptional regulator, DNA helicase and as key factors in site-specific integration of the viral genome. Regulates host PKA activity by interacting with host PRKX as a mechanism of interfering with helper virus propagation and promoting its own replication. Inhibits the host cell cycle G1/S, S and G2/M transitions. These arrests may provide essential cellular factors for viral DNA replication.
种属	AAV-2
表达系统	E. coli
标签	N-MBP, C-6xHis
蛋白编号	Q89268
别名	Rep78,Protein Rep78
氨基酸序列	MPGFYEIVIKVPSDLDGHLPGISDSFVNWVAEKEWELPPDSDMDLNLIEQAPLTVAEKLQRDFLTEWRRVSKAPEALFFVQFEKGESYFHMHVLVETTGVKSMVLGRFLSQIREKLIQRIYRGIEPTLPNWFAVTKTRNGAGGGNKVVDECYIPNYLLPKTQPELQWAWTNMEQYLSACLNLTERKRLVAQHLTHVSQTQEQNKENQNPNSDAPVIRSKTSARYMELVGWLVDKGITSEKQWIQEDQASYISFNAASNSRSQIKAALDNAGKIMSLTKTAPDYLVGQQPVEDISSNRIYKILELNGYDPQYAASVFLGWATKKFGKRNTIWLFGPATTGKTNIAEAIAHTVPFYGCVNWTNENFPFNDCVDKMVIWWEEGKMTAKVVESAKAILGGSKVRVDQKCKSSAQIDPTPVIVTSNTNMCAVIDGNSTTFEHQQPLQDRMFKFELTRRLDHDFGKVTKQEVKDFFRWAKDHVVEVEHEFYVKKGGAKKRPAPSDADISEPKRVRESVAQPSTSDAEASINYADRYQNKCSRHVGMNLMLFPCRQCERMNQNSNICFTHGQKDCLECFPVSESQPVSVVKKAYQKLCYIHHIMGKVPDACTACDLVNVDLDDCIFEQ
蛋白构建	1-621 aa
蛋白纯度	> 85% as determined by SDS-PAGE.
分子量	114.3 kDa (predicted)
内毒素	< 1.0 EU/μg of the protein as determined by the LAL method.
缓冲液	If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
复溶方法	Reconstitute the lyophilized protein in sterile deionized water. The product concentration should not be less than 100 μg/mL. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
存储	Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
运输方式	In general, Lyophilized powders are shipping with blue ice. Solutions are shipping with dry ice.
研究背景	Plays an essential role in the initiation of viral DNA synthesis. Binds specifically to an inverted terminal repeat element (ITR) on the 3' and 5' ends of the viral DNA, where it cleaves a site specifically to generate a priming site for initiation of the synthesis of a complementary strand. Plays also a role as transcriptional regulator, DNA helicase and as key factors in site-specific integration of the viral genome. Regulates host PKA activity by interacting with host PRKX as a mechanism of interfering with helper virus propagation and promoting its own replication. Inhibits the host cell cycle G1/S, S and G2/M transitions. These arrests may provide essential cellular factors for viral DNA replication.