购物车
  • 全部删除
  • TargetMol
    您的购物车当前为空

ACVR2B Protein, Cynomolgus, Recombinant

产品编号 TMPY-04044

ACVR2B Protein, Cynomolgus, Recombinant is expressed in HEK293 mammalian cells. The predicted molecular weight is 14.1 kDa and the accession number is G7MJ10.

ACVR2B Protein, Cynomolgus, Recombinant

ACVR2B Protein, Cynomolgus, Recombinant

产品编号 TMPY-04044
ACVR2B Protein, Cynomolgus, Recombinant is expressed in HEK293 mammalian cells. The predicted molecular weight is 14.1 kDa and the accession number is G7MJ10.
规格价格库存数量
100 μg
¥ 5,100
5日内发货
大包装 & 定制
加入购物车
TargetMol 的所有产品仅用作科学研究或药证申报,不能被用于人体,我们不向个人提供产品和服务。请您遵守承诺用途,不得违反法律法规规定用于任何其他用途。
实验操作小课堂
常见问题解答
准备进行动物实验,收到大包装粉末后,如何分装溶解?
根据配制出来的溶液是不是澄清的确定一次性能配多少:如果配制出来是澄清溶液,可以一次性多配制一些,储存于4度冰箱,大概一周配一次,放久了可能会失效;如果配制出来是混悬液,建议每次使用的时候都现配现用。
期货产品没有纯度啊?这个纯度在哪里看
期货产品的话,是没有纯度信息,也没有质检信息的。有了现货之后我们会进行质检,测出试剂的纯度和结构等相关信息,并且展示在官网的活性描述上方。
如何设置母液浓度?
母液浓度需低于官网给出的溶解度,在这个范围内,根据工作液浓度设置母液浓度,细胞实验中,建议母液浓度设定在工作液浓度的 1000 倍以上。
收到化合物后,是否需要称量后配制储备液?
对于小包装,如 1 mg、5 mg、10 mg 等,不建议二次称量。这样会导致化合物损耗,且无法估算。TargetMol 所有产品原装进口,精准称量,您收到后可直接添加相应量溶剂。 对于大包装,如果您需要分批次实验,可每次称量后配制储备液,建议您 10 mg 起称,称量的误差会小一些;当然条件允许,也可以先配制成高浓度母液,分装备用。
抑制剂母液的储存方式?
母液配置完成后,一般储存于 -80℃,可以存 1 年以上。建议多分装几管,避免反复冻融,常用的存于 4 度,可放一周以上。
查看更多

产品信息

生物活性
Measured by its ability to neutralize Activin-mediated inhibition on MPC11 cell proliferation. The ED50 for this effect is typically 0.2-0.6 µg/mL in the presence of 10 ng/mL recombinant Activin A.
产品描述
ACVR2B Protein, Cynomolgus, Recombinant is expressed in HEK293 mammalian cells. The predicted molecular weight is 14.1 kDa and the accession number is G7MJ10.
种属
Cynomolgus
表达系统
HEK293 Cells
标签Tag Free
蛋白编号G7MJ10
别名
activin A receptor, type IIB
蛋白构建
A DNA sequence encoding the cynomolgus ACVR2B (EHH16502.1) (Ser28-Thr143) was expressed with six amino acids (LEVLFQ) at the C-terminus. Predicted N terminal: Ser 28
蛋白纯度
> 95 % as determined by SDS-PAGE
分子量14.1 kDa (predicted); 34 kDa (reducing conditions)
内毒素< 1.0 EU/μg of the protein as determined by the LAL method.
缓冲液Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
复溶方法
A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
存储
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
运输方式In general, Lyophilized powders are shipping with blue ice.
研究背景
ACVR2A and ACVR2B are two activin type II receptors. ACVR2B is integral to the activin and myostatin signaling pathway. Ligands such as activin and myostatin bind to ACVR2A and ACVR2B. Myostatin, a negative regulator of skeletal muscle growth, is regarded as a potential therapeutic target and binds to ACVR2B effectively, and to a lesser extent, to ACVR2A. The structure of human ACVR2B kinase domain in complex with adenine establishes the conserved bilobal architecture consistent with all other catalytic kinase domains. Haplotype structure at the ACVR2B and follistatin loci may contribute to interindividual variation in skeletal muscle mass and strength. Defects in ACVR2B are a cause of left-right axis malformations.

参考文献

SCI 文献

计算器

  • 复溶 计算器
  • 重组蛋白稀释 计算器
  • 比活力 计算器

技术支持

请阅读 重组蛋白用户指南 了解更多具体信息.