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14-3-3 sigma Protein, Human, Recombinant

产品编号 TMPY-01275

14-3-3 sigma Protein, Human, Recombinant is expressed in E. coli expression system. The predicted molecular weight is 28 kDa and the accession number is P31947-1.

14-3-3 sigma Protein, Human, Recombinant

14-3-3 sigma Protein, Human, Recombinant

产品编号 TMPY-01275
14-3-3 sigma Protein, Human, Recombinant is expressed in E. coli expression system. The predicted molecular weight is 28 kDa and the accession number is P31947-1.
规格价格库存数量
100 μg
¥ 4,460
5日内发货
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产品信息

生物活性
Activity testing is in progress. It is theoretically active, but we cannot guarantee it. If you require protein activity, we recommend choosing the eukaryotic expression version first.
产品描述
14-3-3 sigma Protein, Human, Recombinant is expressed in E. coli expression system. The predicted molecular weight is 28 kDa and the accession number is P31947-1.
种属
Human
表达系统
E. coli
标签Tag Free
蛋白编号P31947-1
别名
YWHAS,stratifin,14-3-3 σ
蛋白构建
A DNA sequence encoding the human SFN (NP_006133.1) (Met 1-Ser 248) was expressed, with two additional amino acids (Gly & Pro) at the N-terminus. Predicted N terminal: Gly
蛋白纯度
> 97 % as determined by SDS-PAGE
分子量28 kDa (predicted); 28 kDa (reducing conditions)
内毒素Please contact us for more information.
缓冲液Supplied as sterile 20 mM Tris, 150 mM NaCl, 10 mM GSH, 25% glycerol, pH 8.0.
复溶方法
A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
存储
It is recommended to store the product under sterile conditions at -20°C to -80°C. Samples are stable for up to 12 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
运输方式In general, Lyophilized powders are shipping with blue ice. Solutions are shipping with dry ice.
研究背景
14-3-3 protein sigma (YWHAS), also known as stratifin (SFN) and epithelial cell marker protein 1, is a member of the 14-3-3 proteins which are a family of conserved regulatory molecules expressed in all eukaryotic cells. The name 14-3-3 refers to the particular elution and migration pattern of these proteins on DEAE-cellulose chromatography and starch-gel electrophoresis. The 14-3-3 proteins eluted in the 14th fraction of bovine brain homogenate and were found on positions 3.3 of subsequent electrophoresis. There are seven genes that encode 14-3-3s in most mammals. 14-3-3 proteins have been identified as adapter proteins implicated in the regulation of a large spectrum of both general and specialized signaling pathways. More than 1 signaling proteins have been reported as 14-3-3 ligands including kinases, phosphatases, and transmembrane receptors, and the binding generally results in the modulation of the activity of the binding partner. YWHAE exists as a homodimer and present mainly in tissues enriched in stratified squamous keratinising epithelium. YWHAS has been repoted to interact with KRT17 and GAB2, and may regulate protein synthesis and epithelial cell growth by stimulating Akt/mTOR pathway upon binding to KRT17. Additionally, YWHAS (SFN) may also act as a p53-regulated inhibitor of G2/M progression.

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