Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Triphenyl Compound A (TPh A) is an effective inhibitor of nuclear protein pirin, showing specific binding to pirin with a Ki value of 0.6 uM. Its mechanism of action involves disturbing the formation of the bcl3–pirin complex. TPh A serves as a valuable small molecule tool for the regulation of pirin in cellular environments.
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
2 mg | ¥ 497 | 5日内发货 | ||
5 mg | ¥ 869 | 5日内发货 | ||
1 mL * 10 mM (in DMSO) | ¥ 913 | 5日内发货 |
产品描述 | Triphenyl Compound A (TPh A) is an effective inhibitor of nuclear protein pirin, showing specific binding to pirin with a Ki value of 0.6 uM. Its mechanism of action involves disturbing the formation of the bcl3–pirin complex. TPh A serves as a valuable small molecule tool for the regulation of pirin in cellular environments. |
体外活性 | The Pirin is a nuclear protein with the nuclear factor I/CCAAT box transcription factor (NFI/CTF). Pirin proteins are highly conserved between mammals, plants, fungi, and prokaryotic organisms and are considered to belong to the cupin superfamily.TPh A (20 μM; 5 hours) reduces the amount of pirin-bound Bcl3 and inhibits the interaction between pirin and Bcl3 in HEK293T cells in a glutathione S-transferase (GST) pulldown assay (HEK293T cells are transfected with vectors that encoded bcl3-Myc and pirin-His6 for 43 h of transfection)[1].TPh A (0-100 μM; 48 hours) does not exert any potent cytotoxic activity against many human cancer cell lines, it against MCF7, MDA-MB231, HeLa, DU145, HepG2, A549, HT1080, WM266-4, and SK-MEL-28 cells with IC 50 values >50 μM, and exhibits IC 50 values of 27 μM, 20 μM and 26 μM for PC3 HL60 and HT29 cells, respectively[1].TPhA (0-50 μM; 48 hours) inhibits cell migration in WM266-4 cells, SK-MEL-28 cell in a concentration-dependent manner[1]. Cell Viability Assay[1]Cell Line: MCF7, MDA-MB231, HeLa, DU145, HepG2, A549, HT1080, WM266-4, and SK-MEL-28 cells Concentration: 0-100 μM Incubation Time: 48 hours Result: Did not exert any potent cytotoxic activity against many human cancer cell lines. |
别名 | Triphenyl Compound A |
分子量 | 399.52 |
分子式 | C21H21NO3S2 |
CAS No. | 21306-65-0 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
TPh A 21306-65-0 Triphenyl Compound A Inhibitor inhibitor inhibit