N4-Acetylcytidine-13C5 is a 13C-labeled version of N4-acetylcytidine (N4A), an endogenous nucleoside metabolite produced from tRNA degradation. It is synthesized by enzymes such as N-acetyltransferase 10. N4-acetylcytidine may play a role in maintaining the activation of the NLRP3 inflammasome by inducing the expression and release of HMGB1. Additionally, it influences the stability and translation efficiency of mRNA, tRNA, and rRNA (e.g., enterovirus71RNA) through modification. This compound is utilized in studies related to cancer, neuroinflammatory diseases, viral infections, and obesity.