Multi-target kinase-IN-9 is a multi-target enzyme inhibitor with antiproliferative and antiangiogenic activity, showing significant selectivity for liver cancer cells. By extensively binding to active sites or ATP-binding regions of several key enzymes, such as DNA polymerase β and pyruvate kinase M2 (PKM2), it disrupts DNA repair and replication, glycolysis, chromatin dynamics, and transcriptional programs, blocking cancer stem cell self-renewal. This compound induces genomic instability, lysosomal dysfunction, and autophagic flux interruption, leading to tumor cell death. It effectively inhibits tumor proliferation, invasion, metastasis, and angiogenesis, significantly reducing tumor volume in xenograft models. Multi-target kinase-IN-9 is applicable in liver cancer research.