MB 543 DBCO is a fluorescent agent with a terminal DBCO group. The DBCO group is commonly utilized in copper-free click chemistry due to its strain-induced high reactivity. MB 543 alkyne is a highly hydrophilic, water-soluble orange fluorescent dye, exhibiting a maximum absorption wavelength of 543 nm and an emission wavelength of 566 nm. This compound remains stable across a pH range of pH 3 to pH 10.